- Express System: E.coli
- Product Tag: No Tag
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Human LIF, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human LIF R, His Tag with the EC50 of 0.30ug/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Ser23-Phe202
Product Description |
Recombinant Human LIF Protein is expressed from E.coli without tag.It contains Ser23-Phe202. |
Product Background |
Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether the level of LIF in embryo culture medium can be used as a marker for clinical pregnancy. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
LIF |
Synonyms |
LIF; HILDA; MLPLI; CDF; DIA |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser23-Phe202 |
Molecular Weight |
The protein has a predicted MW of 19.86 kDa same as Bis-Tris PAGE result. |
Activity |
Immobilized Human LIF, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human LIF R, His Tag with the EC50 of 0.30ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
No Tag |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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