Human LIF Protein (LIF-HM001)

Express System: HEK293
Product Tag: No Tag
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human LIF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human LIF R, hFc Tag with the EC50 of 55.9ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ser23-Phe202

Catalog: LIF-HM001

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Product Description	Recombinant Human LIF Protein is expressed from HEK293 without tag.It contains Ser23-Phe202.
Product Background	Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether the level of LIF in embryo culture medium can be used as a marker for clinical pregnancy.
Product Category	Recombinant Protein

Target	LIF
Synonyms	LIF; HILDA; MLPLI; CDF; DIA
Gene/Protein ID	P15018-1
Species	Human
Exact Sequence	Ser23-Phe202
Molecular Weight	The protein has a predicted MW of 19.7 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human LIF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human LIF R, hFc Tag with the EC50 of 55.9ng/ml determined by ELISA. See testing image for detail.
Product Tag	No Tag
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human LIF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human LIF R, hFc Tag with the EC50 of 55.9ng/ml determined by ELISA.
	Determined by its dose-dependent ability to induce reporter gene in 293T-STAT3-Luc2 reporter cells. The ED50-for this effect is 1-5 ng/mL.
	The purity of Human LIF is greater than 95% as determined by SEC-HPLC.
	Human LIF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.