Name: Human LIF Protein (LIF-HM001)
Brand: KactusBio
SKU: LIF-HM001-100UG
Price: 346.5 USD
Availability: InStock

Express System: HEK293
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human LIF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human LIF R, hFc Tag with the EC50 of 0.10ug/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Ser23-Phe202

Catalog #LIF-HM001

Size

100UG

500UG

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Human LIF Protein (LIF-HM001) Summary

Product Description	Recombinant Human LIF Protein is expressed from HEK293 without tag.It contains Ser23-Phe202.
Product Background	Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether the level of LIF in embryo culture medium can be used as a marker for clinical pregnancy.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human LIF Protein (LIF-HM001) Specifications/Details

Protein	LIF
Synonyms	LIF; HILDA; MLPLI; CDF; DIA
Accession	P15018-1
Species	Human
Biotinylated	no
Amino Acid Range	Ser23-Phe202
Molecular Weight	The protein has a predicted MW of 19.7 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human LIF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human LIF R, hFc Tag with the EC50 of 0.10ug/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	No Tag
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human LIF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human LIF R, hFc Tag with the EC50 of 0.10ug/ml determined by ELISA (QC Test).
	Human LIF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Determined by its dose-dependent ability to induce reporter gene in 293T-STAT3-Luc2 reporter cells. The ED50-for this effect is 0.2-2 ng/mL.
	The purity of Human LIF is greater than 95% as determined by SEC-HPLC.