Product Description |
Recombinant Human LIF Protein is expressed from HEK293 without tag.It contains Ser23-Phe202. |
Product Background |
Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether the level of LIF in embryo culture medium can be used as a marker for clinical pregnancy. |
Product Category |
Recombinant Protein |
Target |
LIF |
Synonyms |
LIF; HILDA; MLPLI; CDF; DIA |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser23-Phe202 |
Molecular Weight |
The protein has a predicted MW of 19.7 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human LIF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human LIF R, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
No Tag |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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|
Immobilized Human LIF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human LIF R, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA (QC Test). |
|
|
Human LIF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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|
Determined by its dose-dependent ability to induce reporter gene in 293T-STAT3-Luc2 reporter cells. The ED50-for this effect is 1-5 ng/mL. |
|
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The purity of Human LIF is greater than 95% as determined by SEC-HPLC. |
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