Human LIF Protein (LIF-HM001)

  • Express System: Expi293
  • Product Tag: No Tag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LIF at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human LIF R, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ser23-Phe202

Catalog: LIF-HM001

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Product Description Recombinant Human LIF Protein is expressed from Expi293 without tag.It contains Ser23-Phe202.
Product Category Recombinant Protein
Target LIF
Synonyms LIF; HILDA; MLPLI; CDF; DIA
Gene/Protein ID P15018-1
Species Human
Exact Sequence Ser23-Phe202
Molecular Weight The protein has a predicted MW of 19.7 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human LIF at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human LIF R, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. See testing image for detail.
Product Tag No Tag
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human LIF at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human LIF R, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA.
The purity of Human LIF is greater than 95% as determined by SEC-HPLC.
Human LIF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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