Human LIF R/CD118 Protein (LIF-HM10R)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LIF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human LIF R, His Tag with the EC50 of 0.30ug/ml determined by ELISA (QC Test). The affinity constant of 0.662 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gln45-Ser833

Catalog: LIF-HM10R

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Product Description Recombinant Human LIF R/CD118 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln45-Ser833.

Product Background

The leukemia inhibitory factor receptor (LIF-R) subunit is a component of cell-surface receptor complexes for the multifunctional cytokines, LIF, cardiotrophin-1, ciliary neurotrophic factor, and human oncostatin M. The structure of the human LIF-R gene is similar to that of the mouse gene. The transmembrane receptor is encoded by 19 exons.

Product Category

Recombinant Protein

Target

LIF R/CD118
Synonyms FLJ99923; SJS2; STWS; SWS; LIFR; CD118; FLJ98106
Accession

Species

Human

Amino Acid Range

Gln45-Ser833
Molecular Weight The protein has a predicted MW of 90.5 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human LIF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human LIF R, His Tag with the EC50 of 0.30ug/ml determined by ELISA (QC Test). The affinity constant of 0.662 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human LIF R, His Tag immobilized on CM5 Chip can bind Human Oncostatin M, His Tag with an affinity constant of 0.662 uM as determined in SPR assay (Biacore T200).
Immobilized Human LIF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human LIF R, His Tag with the EC50 of 0.30ug/ml determined by ELISA (QC Test).
The purity of Human LIF R/CD118 is greater than 95% as determined by SEC-HPLC.
Human LIF R/CD118 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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