Human LIF R/CD118 Protein (LIF-HM10R)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LIF at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human LIF R, His Tag with the EC50 of 0.75μg/ml determined by ELISA. The affinity constant of 0.662 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gln45-Ser833

Catalog: LIF-HM10R

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Product Description Recombinant Human LIF R/CD118 Protein is expressed from Expi293 with His tag at the C-terminal.It contains Gln45-Ser833.
Product Category Recombinant Protein
Target LIF R/CD118
Synonyms FLJ99923; SJS2; STWS; SWS; LIFR; CD118; FLJ98106
Gene/Protein ID P42702-1
Species Human
Exact Sequence Gln45-Ser833
Molecular Weight The protein has a predicted MW of 90.5 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human LIF at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human LIF R, His Tag with the EC50 of 0.75μg/ml determined by ELISA. The affinity constant of 0.662 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human LIF R, His Tag immobilized on CM5 Chip can bind Human Oncostatin M, His Tag with an affinity constant of 0.662 μM as determined in SPR assay (Biacore T200).
Immobilized Human LIF at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human LIF R, His Tag with the EC50 of 0.75μg/ml determined by ELISA.
The purity of Human LIF R/CD118 is greater than 95% as determined by SEC-HPLC.
Human LIF R/CD118 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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