Name: Human LIF R/CD118 Protein (LIF-HM10R)
Brand: KactusBio
SKU: LIF-HM10R-100UG
Price: 346.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human LIF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human LIF R, hFc Tag with the EC50 of 0.10ug/ml determined by ELISA. The affinity constant of 0.662 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gln45-Ser833

Catalog #LIF-HM10R

Size

100UG

500UG

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Human LIF R/CD118 Protein (LIF-HM10R) Summary

Product Description	Recombinant Human LIF R/CD118 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln45-Ser833.
Product Background	The leukemia inhibitory factor receptor (LIF-R) subunit is a component of cell-surface receptor complexes for the multifunctional cytokines, LIF, cardiotrophin-1, ciliary neurotrophic factor, and human oncostatin M. The structure of the human LIF-R gene is similar to that of the mouse gene. The transmembrane receptor is encoded by 19 exons.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human LIF R/CD118 Protein (LIF-HM10R) Specifications/Details

Protein	LIF R/CD118
Synonyms	FLJ99923; SJS2; STWS; SWS; LIFR; CD118; FLJ98106
Accession	P42702-1
Species	Human
Biotinylated	no
Amino Acid Range	Gln45-Ser833
Molecular Weight	The protein has a predicted MW of 90.5 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human LIF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human LIF R, hFc Tag with the EC50 of 0.10ug/ml determined by ELISA. The affinity constant of 0.662 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human LIF R, His Tag immobilized on CM5 Chip can bind Human Oncostatin M, His Tag with an affinity constant of 0.662 uM as determined in SPR assay (Biacore T200).
	Immobilized Human LIF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human LIF R, hFc Tag with the EC50 of 0.10ug/ml determined by ELISA.
	Human LIF R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human LIF R is greater than 95% as determined by SEC-HPLC.