• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LIF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human LIF R, His Tag with the EC50 of 0.15ug/ml determined by ELISA. The affinity constant of 0.662 uM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Gln45-Ser833
Catalog #LIF-HM10R
Price
Regular price $346.50
Regular price Sale price $346.50
/
Size
Avantor VWR Fisher Scientific

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Human LIF R/CD118 Protein (LIF-HM10R) Summary

Product Description

 Recombinant Human LIF R/CD118 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln45-Ser833.

Product Background

 The leukemia inhibitory factor receptor (LIF-R) subunit is a component of cell-surface receptor complexes for the multifunctional cytokines, LIF, cardiotrophin-1, ciliary neurotrophic factor, and human oncostatin M. The structure of the human LIF-R gene is similar to that of the mouse gene. The transmembrane receptor is encoded by 19 exons.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human LIF R/CD118 Protein (LIF-HM10R) Specifications/Details

Protein
 LIF R/CD118
Synonyms
 FLJ99923; SJS2; STWS; SWS; LIFR; CD118; FLJ98106
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gln45-Ser833
Molecular Weight
 The protein has a predicted MW of 90.5 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human LIF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human LIF R, His Tag with the EC50 of 0.15ug/ml determined by ELISA. The affinity constant of 0.662 uM as determined in SPR assay (Biacore T200).
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human LIF R, His Tag immobilized on CM5 Chip can bind Human Oncostatin M, His Tag with an affinity constant of 0.662 uM as determined in SPR assay (Biacore T200). Human LIF R, His Tag immobilized on CM5 Chip can bind Human Oncostatin M, His Tag with an affinity constant of 0.662 uM as determined in SPR assay (Biacore T200).
Immobilized Human LIF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human LIF R, His Tag with the EC50 of 0.15ug/ml determined by ELISA. Immobilized Human LIF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human LIF R, His Tag with the EC50 of 0.15ug/ml determined by ELISA.
Human LIF R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human LIF R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human LIF R is greater than 95% as determined by SEC-HPLC. The purity of Human LIF R is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...