Name: Human LIGHT/TNFSF14 Protein (LGT-HM231)
Brand: KactusBio
SKU: LGT-HM231-100UG
Price: 330.0 USD
Availability: InStock

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human HVEM, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human LIGHT, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Asp74-Val240

Catalog #LGT-HM231

Size

100UG

500UG

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Product Description	Recombinant Human LIGHT/TNFSF14 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Asp74-Val240.
Product Background	LIGHT, also known as tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. It is recognized by herpesvirus entry mediator (HVEM), as well as decoy receptor 3.This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells.
Product Category	Recombinant Protein / Antibody discovery

Protein	LIGHT/TNFSF14
Synonyms	CD258; TNFSF14; HVEM-L; LIGHT; LTg;-TR2
Accession	O43557-1
Species	Human
Biotinylated	no
Amino Acid Range	Asp74-Val240
Molecular Weight	The protein has a predicted MW of 45.5 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human HVEM, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human LIGHT, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human HVEM, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human LIGHT, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA.
	Human LIGHT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human LIGHT is greater than 95% as determined by SEC-HPLC.