Human LIGHT/TNFSF14 Protein (LGT-HM231)

  • Express System: Expi293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human HVEM, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human LIGHT, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Asp74-Val240

Catalog: LGT-HM231

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Product Description Recombinant Human LIGHT/TNFSF14 Protein is expressed from Expi293 with hFc tag at the N-terminal.It contains Asp74-Val240.
Product Category Recombinant Protein
Target LIGHT/TNFSF14
Synonyms CD258; TNFSF14; HVEM-L; LIGHT; LTg; TR2
Gene/Protein ID O43557-1
Species Human
Exact Sequence Asp74-Val240
Molecular Weight The protein has a predicted MW of 45.5 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human HVEM, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human LIGHT, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA. See testing image for detail.
Product Tag N-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human HVEM, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human LIGHT, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA.
The purity of Human LIGHT is greater than 95% as determined by SEC-HPLC.
Human LIGHT on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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