Product Description |
Recombinant Human LIGHT/TNFSF14 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Asp74-Val240. |
Product Background |
LIGHT, also known as tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. It is recognized by herpesvirus entry mediator (HVEM), as well as decoy receptor 3.This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. |
Product Category |
Recombinant Protein |
Target |
LIGHT/TNFSF14 |
Synonyms |
CD258; TNFSF14; HVEM-L; LIGHT; LTg;-TR2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asp74-Val240 |
Molecular Weight |
The protein has a predicted MW of 45.5 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human HVEM, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human LIGHT, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human HVEM, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human LIGHT, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA. |
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Human LIGHT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human LIGHT is greater than 95% as determined by SEC-HPLC. |
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