Human LIGHT/TNFSF14 Protein (LGT-HM231)
Human LIGHT/TNFSF14 Protein (LGT-HM231)
  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human HVEM, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human LIGHT, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Asp74-Val240
Catalog #LGT-HM231
Regular price $330.00
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Product Description

Recombinant Human LIGHT/TNFSF14 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Asp74-Val240.

Product Background

LIGHT, also known as tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. It is recognized by herpesvirus entry mediator (HVEM), as well as decoy receptor 3.This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells.

Product Category

Recombinant Protein / Antibody discovery

Protein

LIGHT/TNFSF14

Synonyms

CD258; TNFSF14; HVEM-L; LIGHT; LTg;-TR2

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Asp74-Val240

Molecular Weight

The protein has a predicted MW of 45.5 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human HVEM, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human LIGHT, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA. See testing image for detail.

Product Tag

N-hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human HVEM, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human LIGHT, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA. Immobilized Human HVEM, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human LIGHT, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA.
Human LIGHT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human LIGHT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human LIGHT is greater than 95% as determined by SEC-HPLC. The purity of Human LIGHT is greater than 95% as determined by SEC-HPLC.

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