Human LIGHT/TNFSF14 Trimer Protein (LGT-HM132)

  • Express System: Expi293
  • Product Tag: N-His-Flag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LIGHT Trimer, His tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 37.5ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ser89-Val240

Catalog: LGT-HM132

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Product Description Recombinant Human LIGHT/TNFSF14 Trimer Protein is expressed from Expi293 with His tag and Flag tag at the N-terminal.It contains Ser89-Val240 Trimer.
Product Category Recombinant Protein
Target LIGHT/TNFSF14 Trimer
Synonyms CD258; TNFSF14; HVEM-L; LIGHT; LTg; TR2
Gene/Protein ID O43557-1
Species Human
Exact Sequence Ser89-Val240
Molecular Weight The protein has a predicted MW of 52.4 kDa. Due to glycosylation, the protein migrates to 53-140 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human LIGHT Trimer, His tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 37.5ng/ml determined by ELISA. See testing image for detail.
Product Tag N-His-Flag
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human LIGHT Trimer, His tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 37.5ng/ml determined by ELISA.
The purity of Human LIGHT Trimer is greater than 95% as determined by SEC-HPLC.
Human LIGHT Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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