Human LIGHT/TNFSF14 Trimer Protein (LGT-HM132)
Human LIGHT/TNFSF14 Trimer Protein (LGT-HM132)
Human LIGHT/TNFSF14 Trimer Protein (LGT-HM132)
Human LIGHT/TNFSF14 Trimer Protein (LGT-HM132)
Human LIGHT/TNFSF14 Trimer Protein (LGT-HM132)
  • Express System: HEK293
  • Product Tag: N-His-Flag
  • Purity: >90% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Human LIGHT Trimer, His tag at 5ug/ml (100 ul/well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 21.5ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Ser89-Val240
Catalog #LGT-HM132
Regular price $297.00
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Product Description

Recombinant Human LIGHT/TNFSF14 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.; It contains Ser89-Val240 Trimer.

Product Background

LIGHT, also known as tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. It is recognized by herpesvirus entry mediator (HVEM), as well as decoy receptor 3. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells.

Product Category

Recombinant Protein / Antibody discovery

Protein

LIGHT/TNFSF14 Trimer

Synonyms

CD258; TNFSF14; HVEM-L; LIGHT; LTg;-TR2

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Ser89-Val240

Molecular Weight

The protein has a predicted MW of 52.4 kDa. Due to glycosylation, the protein migrates to 53-140 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.

Activity

Immobilized Human LIGHT Trimer, His tag at 5ug/ml (100 ul/well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 21.5ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

N-His-Flag

Expression System

HEK293

Purity

>90% as determined by Bis-Tris PAGE;>95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human LIGHT Trimer, His tag at 5ug/ml (100 ul/well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 21.5ng/ml determined by ELISA (QC Test). Immobilized Human LIGHT Trimer, His tag at 5ug/ml (100 ul/well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 21.5ng/ml determined by ELISA (QC Test).
Human LIGHT Trimer on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 90%. Human LIGHT Trimer on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 90%.
The purity of Human LIGHT Trimer is greater than 95% as determined by SEC-HPLC. The purity of Human LIGHT Trimer is greater than 95% as determined by SEC-HPLC.

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