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Human LIGHT/TNFSF14 Trimer Protein (LGT-HM132) Summary
Product Description |
Recombinant Human LIGHT/TNFSF14 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.; It contains His90-Val240 trimer design. |
Product Background |
LIGHT, also known as tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. It is recognized by herpesvirus entry mediator (HVEM), as well as decoy receptor 3. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human LIGHT/TNFSF14 Trimer Protein (LGT-HM132) Specifications/Details
Protein |
LIGHT/TNFSF14 |
Synonyms |
CD258; TNFSF14; HVEM-L; LIGHT; LTg;-TR2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
His90-Val240 |
Molecular Weight |
The protein has a predicted MW of 52.4 kDa. Due to glycosylation, the protein migrates to 53-140 kDa under Non reduced (N) condition based on Bis-Tris PAGE result. |
Activity |
Immobilized Human LIGHT Trimer, His tag at 5ug/ml (100 ul/well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 21.5ng/ml determined by ELISA (QC Test). |
Product Tag |
N-His-Flag |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 90% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human LIGHT Trimer, His tag at 5ug/ml (100 ul/well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 21.5ng/ml determined by ELISA (QC Test). |
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Human LIGHT Trimer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 90%. |
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The purity of Human LIGHT Trimer is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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