Product Description |
Recombinant Human LILRA4/CD85g Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu24-Asn446. |
Product Background |
LILRA4, also known as ILT7 and CD85g, is an approximately 60-70 kDa variably glycosylated transmembrane protein that regulates immune cell activation. Mature human LILRA4 consists of a 423 amino acid (aa) extracellular domain (ECD) with four immunoglobulin-like domains, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain.LILRA4 function coreceptor to limit the innate immune responses to viral infections; signaling occurs via FCER1G (PubMed:16735691, PubMed:19564354). Down-regulates the production of IFNA1, IFNA2, IFNA4, IFNB1 and TNF by plasmacytoid dendritic cells that have been exposed to influenza virus or cytidine-phosphate-guanosine (CpG) dinucleotides, indicating it functions as negative regulator of TLR7 and TLR9 signaling cascades. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Protein |
LILRA4/CD85g |
Synonyms |
CD85g; ILT7; ILT-7; ILT7MGC129598; LILRA4; MGC129597 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu24-Asn446 |
Molecular Weight |
The protein has a predicted MW of 49.5 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Human LILRA4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
|
|
The purity of Human LILRA4 is greater than 95% as determined by SEC-HPLC. |
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