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Human LILRB1/CD85j/ILT2 Domain1&2-Protein (LIL-HM11D) Summary
Product Description |
Recombinant Human LILRB1/CD85j/IL-T2 Domain1&2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly24-Gly221. |
Product Background |
LILRB1, also known as CD85j and IL-T2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family. Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs.LILRB1 is a receptor for class I MHC antigens. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Human LILRB1/CD85j/ILT2 Domain1&2-Protein (LIL-HM11D) Specifications/Details
Protein |
LILRB1/CD85j/ILT2 |
Synonyms |
ILT2; ILT-2; ILT2FLJ37515; LILRB1; LIR1; MIR7; CD85J; XXbac-BCX85G21.4 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly24-Gly221 |
Molecular Weight |
The protein has a predicted MW of 23.1 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human LILRB1 Domain1&2, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA (QC Test). The affinity constant of 1.9uM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human HLA-G Tetramer captured on CM5 Chip can bind Human LILRB1 Domain1&2, His Tag with an affinity constant of 1.9uM as determined in SPR assay (Biacore T200). |
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Immobilized Human LILRB1 Domain1&2, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA (QC Test). |
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Human LILRB1 Domain1&2 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
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Serial dilutions of Anti-LILRB1 Antibody were added into Biotinylated Human HLA-G Complex Tetramer, His Tag : Human LILRB1 Domain1&2, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 34.6ng/ml. |
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The purity of Human LILRB1 Domain1&2 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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