Name: Human LILRB1/CD85j/ILT2 Domain1&2-Protein (LIL-HM11D)
Brand: KactusBio
SKU: LIL-HM11D-100UG
Price: 231.0 USD
Availability: InStock

Human LILRB1/CD85j/ILT2 Domain1&2-Protein (LIL-HM11D)

Human HLA-G Tetramer captured on CM5 Chip can bind Human LILRB1 Domain1&2, His Tag with an affinity constant of 1.9uM as determined in SPR assay (Biacore T200).

Immobilized Human LILRB1 Domain1&2, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA (QC Test).

Human LILRB1 Domain1&2 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

Serial dilutions of Anti-LILRB1 Antibody were added into Biotinylated Human HLA-G Complex Tetramer, His Tag : Human LILRB1 Domain1&2, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 34.6ng/ml.

The purity of Human LILRB1 Domain1&2 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human LILRB1 Domain1&2, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA (QC Test). The affinity constant of 1.9uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gly24-Gly221

Catalog #LIL-HM11D

Size

100UG

500UG

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Product Description	Recombinant Human LILRB1/CD85j/IL-T2 Domain1&2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly24-Gly221.
Product Background	LILRB1, also known as CD85j and IL-T2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family. Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs.LILRB1 is a receptor for class I MHC antigens.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	LILRB1/CD85j/ILT2 Domain1&2
Synonyms	ILT2; ILT-2; ILT2FLJ37515; LILRB1; LIR1; MIR7; CD85J; XXbac-BCX85G21.4
Accession	Q8NHL6-1
Species	Human
Biotinylated	no
Amino Acid Range	Gly24-Gly221
Molecular Weight	The protein has a predicted MW of 23.1 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human LILRB1 Domain1&2, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA (QC Test). The affinity constant of 1.9uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human HLA-G Tetramer captured on CM5 Chip can bind Human LILRB1 Domain1&2, His Tag with an affinity constant of 1.9uM as determined in SPR assay (Biacore T200).
	Immobilized Human LILRB1 Domain1&2, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA (QC Test).
	Human LILRB1 Domain1&2 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
	Serial dilutions of Anti-LILRB1 Antibody were added into Biotinylated Human HLA-G Complex Tetramer, His Tag : Human LILRB1 Domain1&2, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 34.6ng/ml.
	The purity of Human LILRB1 Domain1&2 is greater than 95% as determined by SEC-HPLC.