Human LILRB1/CD85j/ILT2 Domain1&2-Protein (LIL-HM11D)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LILRB1 Domain1&2, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA (QC Test). The affinity constant of 1.9uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly24-Gly221
Catalog #LIL-HM11D
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Product Description
 Recombinant Human LILRB1/CD85j/IL-T2 Domain1&2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly24-Gly221.
Product Background
 LILRB1, also known as CD85j and IL-T2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family. Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs.LILRB1 is a receptor for class I MHC antigens.
Product Category
 Recombinant Protein
Target
 LILRB1/CD85j/ILT2 Domain1&2
Synonyms
 ILT2; ILT-2; ILT2FLJ37515; LILRB1; LIR1; MIR7; CD85J; XXbac-BCX85G21.4
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly24-Gly221
Molecular Weight
 The protein has a predicted MW of 23.1 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human LILRB1 Domain1&2, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA (QC Test). The affinity constant of 1.9uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human HLA-G Tetramer captured on CM5 Chip can bind Human LILRB1 Domain1&2, His Tag with an affinity constant of 1.9uM as determined in SPR assay (Biacore T200).
Immobilized Human LILRB1 Domain1&2, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA (QC Test).
Human LILRB1 Domain1&2 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
Serial dilutions of Anti-LILRB1 Antibody were added into Biotinylated Human HLA-G Complex Tetramer, His Tag : Human LILRB1 Domain1&2, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 34.6ng/ml.
The purity of Human LILRB1 Domain1&2 is greater than 95% as determined by SEC-HPLC.

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