Human LILRB1/CD85j/ILT2 Domain1&2 Protein (LIL-HM11D)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LILRB1 Domain1&2, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA (QC Test). The affinity constant of 1.9μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gly24-Gly221

Catalog: LIL-HM11D

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Product Description Recombinant Human LILRB1/CD85j/IL-T2 Domain1&2 Protein is expressed from Expi293 with His tag at the C-terminal.It contains Gly24-Gly221.
Product Category Recombinant Protein
Target LILRB1/CD85j/ILT2 Domain1&2
Synonyms ILT2; ILT-2; ILT2FLJ37515; LILRB1; LIR1; MIR7; CD85J; XXbac-BCX85G21.4
Gene/Protein ID Q8NHL6-1
Species Human
Exact Sequence Gly24-Gly221
Molecular Weight The protein has a predicted MW of 23.1 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human LILRB1 Domain1&2, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA (QC Test). The affinity constant of 1.9μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human HLA-G Tetramer captured on CM5 Chip can bind Human LILRB1 Domain1&2, His Tag with an affinity constant of 1.9μM as determined in SPR assay (Biacore T200).
Immobilized Human LILRB1 Domain1&2, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA (QC Test).
Serial dilutions of Anti-LILRB1 Antibody were added into Biotinylated Human HLA-G Complex Tetramer, His Tag : Human LILRB1 Domain1&2, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 34.6ng/ml.
The purity of Human LILRB1 Domain1&2 is greater than 95% as determined by SEC-HPLC.
Human LILRB1 Domain1&2 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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