Human LILRB1/CD85j/ILT2 Protein (LIL-HM2B1)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LILRB1, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-LILRB1 Antibody, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA (QC Test). The affinity constant of 1.49nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly24-Arg457
Catalog #LIL-HM2B1
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Product Description
 Recombinant Human LILRB1/CD85j/ILT2 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly24-Arg457.
Product Background
 LILRB1, also known as CD85j and ILT2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family. Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs.LILRB1 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response.
Product Category
 Recombinant Protein
Target
 LILRB1/CD85j/ILT2
Synonyms
 ILT2; ILT-2; ILT2FLJ37515; LILRB1; LIR1; MIR7; CD85J; XXbac-BCX85G21.4
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly24-Arg457
Molecular Weight
 The protein has a predicted MW of 73.8 kDa. Due to glycosylation, the protein migrates to 78-105 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human LILRB1, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-LILRB1 Antibody, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA (QC Test). The affinity constant of 1.49nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-hFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human LILRB1, hFc Tag captured on Protein A Chip can bind Human HLA-G Tetramer, His Tag with an affinity constant of 1.49nM as determined in SPR assay (Biacore T200).
Immobilized Human LILRB1, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-LILRB1 Antibody, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA (QC Test).
Human LILRB1 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human LILRB1 is greater than 95% as determined by SEC-HPLC.

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