Human LILRB2/CD85d/ILT4 Protein (LIL-HM3B2)

  • Express System: Expi293
  • Product Tag: C-mFc
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human LILRB2, mFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 11.4ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln22-His458

Catalog: LIL-HM3B2

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Product Description Recombinant Human LILRB2/CD85d/ILT4 Protein is expressed from Expi293 with mFc tag at the C-terminal.; It contains Gln22-His458.
Product Category Recombinant Protein
Target LILRB2/CD85d/ILT4
Synonyms CD85d; ILT4; ILT-4; ILT4CD85d; LILRB2; LIR2; MIR10
Gene/Protein ID Q8N423-1
Species Human
Exact Sequence Gln22-His458
Molecular Weight The protein has a predicted MW of 73.6 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human LILRB2, mFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 11.4ng/ml determined by ELISA. See testing image for detail.
Product Tag C-mFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human LILRB2, mFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 11.4ng/ml determined by ELISA.
Serial dilutions of Anti-LILRB2 Antibody, hFc Tag were added into HLA-G Complex Tetramer, His Tag : Human LILRB2, mFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 70.0ng/ml.
The purity of  Human LILRB2 is greater than 95% as determined by SEC-HPLC.
Human LILRB2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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