Product Description |
Recombinant Human LILRB2/CD85d/ILT4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln22-His458. |
Product Background |
The immunoglobulin-like transcript (ILT) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIR). ILT4, also known as LIR-2 and LILRB2, is a type I transmembrane protein expressed primarily on monocytes and dendritic cells (DC). LILRB2 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles. |
Product Category |
Recombinant Protein |
Target |
LILRB2/CD85d/ILT4 |
Synonyms |
CD85d; ILT4; ILT-4; ILT4CD85d; LILRB2; LIR2; MIR10 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln22-His458 |
Molecular Weight |
The protein has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 70-75 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human LILRB2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human LILRB2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test). |
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Human LILRB2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Serial dilutions of Anti-LILRB2 Antibody were added into Human LILRB2, His Tag : Biotinylated HLA-G Complex Tetramer, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 75.3ng/ml. |
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The purity of-Human LILRB2 is greater than 95% as determined by SEC-HPLC. |
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