ELISA binding curve of human LILRB2 protein with anti-LILRB2 antibody, demonstrating concentration-dependent response measured by absorbance at OD450 for KactusBio product validation.
Non-biotinylated Human LILRB2/CD85d/ILT4 Protein (LIL-HM4B2)
Non-biotinylated Human LILRB2/CD85d/ILT4 Protein (LIL-HM4B2)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LILRB2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Gln22-Arg457
Catalog #LIL-HM4B2
Regular price $223.30
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Non-biotinylated Human LILRB2/CD85d/ILT4 Protein (LIL-HM4B2) Summary

Product Description

Recombinant Human LILRB2/CD85d/ILT4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln22-Arg457.

Product Background

The immunoglobulin-like transcript (ILT) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIR). ILT4, also known as LIR-2 and LILRB2, is a type I transmembrane protein expressed primarily on monocytes and dendritic cells (DC). LILRB2 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Non-biotinylated Human LILRB2/CD85d/ILT4 Protein (LIL-HM4B2) Specifications/Details

Protein
LILRB2/CD85d/ILT4
Synonyms
CD85d; ILT4; ILT-4; ILT4CD85d; LILRB2; LIR2; MIR10
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gln22-Arg457
Molecular Weight
The protein has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 70-75 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human LILRB2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human LILRB2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test). Immobilized Human LILRB2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test).
Human LILRB2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human LILRB2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Serial dilutions of Anti-LILRB2 Antibody were added into Human LILRB2, His Tag : Biotinylated HLA-G Complex Tetramer, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 75.3ng/ml. Serial dilutions of Anti-LILRB2 Antibody were added into Human LILRB2, His Tag : Biotinylated HLA-G Complex Tetramer, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 75.3ng/ml.
The purity of-Human LILRB2 is greater than 95% as determined by SEC-HPLC. The purity of-Human LILRB2 is greater than 95% as determined by SEC-HPLC.
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