Name: Human LILRB2/CD85d/ILT4 Protein, Ultra Low Endotoxin (LIL-HM2B2-UL)
Brand: KactusBio
SKU: LIL-HM2B2-UL-100UG
Price: 245.0 USD
Availability: InStock

Human LILRB2/CD85d/ILT4 Protein, Ultra Low Endotoxin (LIL-HM2B2-UL)

Human LILRB2, hFc tag captured on Protein A Chip can bind Human HLA-G Tetramer, His Tag with an affinity constant of 9.20nM as determined in SPR assay (Biacore T200).

Human LILRB2, hFc Tag captured on Protein A chip, can bind Human ANGPTL2, His Tag with an affinity constant of 0.14uM as determined in a SPR assay (Biacore T200).

Immobilized Human LILRB2, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LILRB2 Antibody, hFc Tag with the EC<sub>50</sub> of 14.6ng/ml determined by ELISA (QC Test).

Human LILRB2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of-Human LILRB2 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human LILRB2, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LILRB2 Antibody, hFc Tag with the EC₅₀ of 14.6ng/ml determined by ELISA (QC Test). The affinity constant of 9.20nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gln22-Arg457

Catalog #LIL-HM2B2-UL

Size

100UG

500UG

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Product Description	Recombinant Human LILRB2/CD85d/ILT4 Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Gln22-Arg457.
Product Background	The immunoglobulin-like transcript (ILT) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIR). ILT4, also known as LIR-2 and LILRB2, is a type I transmembrane protein expressed primarily on monocytes and dendritic cells (DC). LILRB2 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	LILRB2/CD85d/ILT4
Synonyms	CD85d; ILT4; ILT-4; ILT4CD85d; LILRB2; LIR2; MIR10
Accession	AAL36991.1
Species	Human
Biotinylated	no
Amino Acid Range	Gln22-Arg457
Molecular Weight	The protein has a predicted MW of 74.1 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human LILRB2, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LILRB2 Antibody, hFc Tag with the EC₅₀ of 14.6ng/ml determined by ELISA (QC Test). The affinity constant of 9.20nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.01 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM Tris, 100mM glycine, 100mM L-arginine, 150mM NaCl (pH 7.5) / PBS, 200mM L-Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human LILRB2, hFc tag captured on Protein A Chip can bind Human HLA-G Tetramer, His Tag with an affinity constant of 9.20nM as determined in SPR assay (Biacore T200).
	Human LILRB2, hFc Tag captured on Protein A chip, can bind Human ANGPTL2, His Tag with an affinity constant of 0.14uM as determined in a SPR assay (Biacore T200).
	Immobilized Human LILRB2, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LILRB2 Antibody, hFc Tag with the EC₅₀ of 14.6ng/ml determined by ELISA (QC Test).
	Human LILRB2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of-Human LILRB2 is greater than 95% as determined by SEC-HPLC.