Name: Human LILRB4/CD85k/ILT3 Protein (LIL-HM2B4)
Brand: KactusBio
SKU: LIL-HM2B4-100UG
Price: 315.0 USD
Availability: InStock

Human LILRB4/CD85k/ILT3 Protein (LIL-HM2B4)

Human LILRB4, hFc Tag captured on CM5 Chip via Protein A can bind Human APOE 4, His Tag with an affinity constant of 74.20 nM as determined in SPR assay (Biacore T200).

Immobilized Human LILRB4, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LILRB4 Antibody, hFc Tag with the EC50 of 27.3ng/ml determined by ELISA (QC Test).

Human LILRB4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human LILRB4 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human LILRB4, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LILRB4 Antibody, hFc Tag with the EC50 of 27.3ng/ml determined by ELISA (QC Test). The affinity constant of 74.20 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gln22-Arg256

Catalog #LIL-HM2B4

Size

100UG

500UG

Order now, ships by:

[{"id":42618373865694,"title":"100UG","option1":"100UG","option2":null,"option3":null,"sku":"LIL-HM2B4-100UG","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"Human LILRB4\/CD85k\/ILT3 Protein (LIL-HM2B4) - 100UG","public_title":"100UG","options":["100UG"],"price":31500,"weight":5,"compare_at_price":null,"inventory_management":"shopify","barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}},{"id":43584284786910,"title":"500UG","option1":"500UG","option2":null,"option3":null,"sku":"LIL-HM2B4-500UG","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"Human LILRB4\/CD85k\/ILT3 Protein (LIL-HM2B4) - 500UG","public_title":"500UG","options":["500UG"],"price":105000,"weight":5,"compare_at_price":null,"inventory_management":"shopify","barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}}]

Product Description	Recombinant Human LILRB4/CD85k/ILT3 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln22-Arg256.
Product Background	LILRB4,also known as CD85k and LIR-5, ILT3,- is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	LILRB4/CD85k/ILT3
Synonyms	HM18; ILT3; ILT-3; LILRB4; LIR5; CD85K
Accession	AAH26309
Species	Human
Biotinylated	no
Amino Acid Range	Gln22-Arg256
Molecular Weight	The protein has a predicted MW of 52.6 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human LILRB4, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LILRB4 Antibody, hFc Tag with the EC50 of 27.3ng/ml determined by ELISA (QC Test). The affinity constant of 74.20 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human LILRB4, hFc Tag captured on CM5 Chip via Protein A can bind Human APOE 4, His Tag with an affinity constant of 74.20 nM as determined in SPR assay (Biacore T200).
	Immobilized Human LILRB4, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LILRB4 Antibody, hFc Tag with the EC50 of 27.3ng/ml determined by ELISA (QC Test).
	Human LILRB4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human LILRB4 is greater than 95% as determined by SEC-HPLC.