Human LILRB4/CD85k/ILT3 Protein (LIL-HM2B4)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human LILRB4, hFc Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LILRB4 Antibody, hFc Tag with the EC50 of 16.1ng/ml determined by ELISA (QC Test). The affinity constant of 74.20 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Gln22-Arg256

Catalog: LIL-HM2B4

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Product Description	Recombinant Human LILRB4/CD85k/ILT3 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln22-Arg256.
Product Category	Recombinant Protein

Target	LILRB4/CD85k/ILT3
Synonyms	HM18; ILT3; ILT-3; LILRB4; LIR5; CD85K
Gene/Protein ID	AAH26309
Species	Human
Exact Sequence	Gln22-Arg256
Molecular Weight	The protein has a predicted MW of 52.6 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human LILRB4, hFc Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LILRB4 Antibody, hFc Tag with the EC50 of 16.1ng/ml determined by ELISA (QC Test). The affinity constant of 74.20 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human LILRB4, hFc Tag captured on CM5 Chip via Protein A can bind Human APOE 4, His Tag with an affinity constant of 74.20 nM as determined in SPR assay (Biacore T200).
	Immobilized Human LILRB4, hFc Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LILRB4 Antibody, hFc Tag with the EC50 of 16.1ng/ml determined by ELISA (QC Test).
	The purity of Human LILRB4 is greater than 95% as determined by SEC-HPLC.
	Human LILRB4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.