Human LILRB4/CD85k/ILT3 Protein (LIL-HM2B4)
Catalog: LIL-HM2B4
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Product Description | Recombinant Human LILRB4/CD85k/ILT3 Protein is expressed from Expi293 with hFc tag at the C-terminal.; It contains Gln22-Arg256. |
Product Category | Recombinant Protein |
Target | LILRB4/CD85k/ILT3 |
Synonyms | HM18; ILT3; ILT-3; LILRB4; LIR5; CD85K |
Gene/Protein ID | AAH26309 |
Species | Human |
Exact Sequence | Gln22-Arg256 |
Molecular Weight | The protein has a predicted MW of 52.6 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human LILRB4, hFc Tag at 0.2μg (100ul/Well) on the plate. Dose response curve for Human Anti-LILRB4 Antibody, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA. The affinity constant of 115.9 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag | C-hFc |
Express System | Expi293 |
Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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