Regular price
$346.50
Order by 4pm ET for delivery on:
Human LILRB4/CD85k/ILT3 Protein (LIL-HM2B4) Summary
Product Description |
Recombinant Human LILRB4/CD85k/ILT3 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gln22-Arg256. |
Product Background |
LILRB4,also known as CD85k and LIR-5, ILT3,- is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Human LILRB4/CD85k/ILT3 Protein (LIL-HM2B4) Specifications/Details
Protein |
LILRB4/CD85k/ILT3 |
Synonyms |
HM18; ILT3; ILT-3; LILRB4; LIR5; CD85K |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln22-Arg256 |
Molecular Weight |
The protein has a predicted MW of 52.6 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human LILRB4, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LILRB4 Antibody, hFc Tag with the EC50 of 27.3ng/ml determined by ELISA (QC Test). The affinity constant of 74.20 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human LILRB4, hFc Tag captured on CM5 Chip via Protein A can bind Human APOE 4, His Tag with an affinity constant of 74.20 nM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Human LILRB4, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LILRB4 Antibody, hFc Tag with the EC50 of 27.3ng/ml determined by ELISA (QC Test). |
|
|
Human LILRB4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human LILRB4 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
