Human LILRB4/CD85k/ILT3 Protein (LIL-HM2B4)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human LILRB4, hFc Tag at 0.2μg (100ul/Well) on the plate. Dose response curve for Human Anti-LILRB4 Antibody, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA. The affinity constant of 115.9 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gln22-Arg256

Catalog: LIL-HM2B4

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Product Description Recombinant Human LILRB4/CD85k/ILT3 Protein is expressed from Expi293 with hFc tag at the C-terminal.; It contains Gln22-Arg256.
Product Category Recombinant Protein
Target LILRB4/CD85k/ILT3
Synonyms HM18; ILT3; ILT-3; LILRB4; LIR5; CD85K
Gene/Protein ID AAH26309
Species Human
Exact Sequence Gln22-Arg256
Molecular Weight The protein has a predicted MW of 52.6 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human LILRB4, hFc Tag at 0.2μg (100ul/Well) on the plate. Dose response curve for Human Anti-LILRB4 Antibody, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA. The affinity constant of 115.9 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human LILRB4, hFc Tag captured on CM5 Chip via Protein A can bind Human APOE, His Tag with an affinity constant of 115.9 nM as determined in SPR assay (Biacore T200).
Immobilized Human LILRB4, hFc Tag at 0.2μg (100ul/Well) on the plate. Dose response curve for Human Anti-LILRB4 Antibody, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA.
The purity of Human LILRB4 is greater than 95% as determined by SEC-HPLC.
Human LILRB4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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