Human LILRB4/CD85k/ILT3 Protein (LIL-HM4B4)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LILRB4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LILRB4 Antibody, hFc Tag with the EC50 of 9.5ng/ml determined by ELISA (QC Test). The affinity constant of 11.87 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gln22-His257

Catalog: LIL-HM4B4

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Product Description Recombinant Human LILRB4/CD85k/ILT3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln22-His257.

Product Background

LILRB4,also known as CD85k and LIR-5, ILT-3,- is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT-3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles.

Product Category

Recombinant Protein

Target

LILRB4/CD85k/ILT3
Synonyms HM18; ILT3; ILT-3; LILRB4; LIR5; CD85K

Gene/Protein ID

AAH26309

Species

Human

Exact Sequence

Gln22-His257
Molecular Weight The protein has a predicted MW of 28.6 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human LILRB4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LILRB4 Antibody, hFc Tag with the EC50 of 9.5ng/ml determined by ELISA (QC Test). The affinity constant of 11.87 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human APOE4, hFc Tag captured on CM5 Chip via Protein A can bind Human LILRB4, His Tag with an affinity constant of 11.87 nM as determined in SPR assay (Biacore T200).
Immobilized Human LILRB4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LILRB4 Antibody, hFc Tag with the EC50 of 9.5ng/ml determined by ELISA (QC Test).
The purity of Human LILRB4 is greater than 95% as determined by SEC-HPLC.
Human LILRB4 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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