Human LILRB4/CD85k/ILT3 Protein (LIL-HM4B4)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human LILRB4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LILRB4 Antibody, hFc Tag with the EC50 of 9.5ng/ml determined by ELISA (QC Test). The affinity constant of 11.87 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gln22-His257

Catalog: LIL-HM4B4

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Product Description	Recombinant Human LILRB4/CD85k/ILT3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln22-His257.
Product Background	LILRB4,also known as CD85k and LIR-5, ILT-3,- is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT-3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles.
Product Category	Recombinant Protein

Target	LILRB4/CD85k/ILT3
Synonyms	HM18; ILT3; ILT-3; LILRB4; LIR5; CD85K
Accession	AAH26309
Species	Human
Amino Acid Range	Gln22-His257
Molecular Weight	The protein has a predicted MW of 28.6 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human LILRB4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LILRB4 Antibody, hFc Tag with the EC50 of 9.5ng/ml determined by ELISA (QC Test). The affinity constant of 11.87 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human APOE4, hFc Tag captured on CM5 Chip via Protein A can bind Human LILRB4, His Tag with an affinity constant of 11.87 nM as determined in SPR assay (Biacore T200).
	Immobilized Human LILRB4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LILRB4 Antibody, hFc Tag with the EC50 of 9.5ng/ml determined by ELISA (QC Test).
	The purity of Human LILRB4 is greater than 95% as determined by SEC-HPLC.
	Human LILRB4 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.