Human LILRB4/CD85k/ILT3 Protein (LIL-HM4B4)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LILRB4, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LILRB4 Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA (QC Test). The affinity constant of 11.87 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gln22-His257
Catalog #LIL-HM4B4
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Product Description
 Recombinant Human LILRB4/CD85k/ILT3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln22-His257.
Product Background
 LILRB4,also known as CD85k and LIR-5, ILT-3,- is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT-3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles.
Product Category
 Recombinant Protein
Target
 LILRB4/CD85k/ILT3
Synonyms
 HM18; ILT3; ILT-3; LILRB4; LIR5; CD85K
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gln22-His257
Molecular Weight
 The protein has a predicted MW of 28.6 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human LILRB4, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LILRB4 Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA (QC Test). The affinity constant of 11.87 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human APOE4, hFc Tag captured on CM5 Chip via Protein A can bind Human LILRB4, His Tag with an affinity constant of 11.87 nM as determined in SPR assay (Biacore T200).
Immobilized Human LILRB4, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LILRB4 Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA (QC Test).
Human LILRB4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human LILRB4 is greater than 95% as determined by SEC-HPLC.

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