• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Amino Acid Range: Cys42-Thr561
Catalog #LIN-HM201
Price
Regular price $000
Regular price Sale price $000
/
Size
Avantor VWR Fisher Scientific

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Human LINGO1 Protein (LIN-HM201) Summary

Product Description

 Recombinant Human LINGO1 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Cys42-Thr561.

Product Background

 LINGO1 is one of four leucine rich repeat and immunoglobin-like (LRRIG) domain-containing proteins predominantly expressed in the central nervous system and may play a role in both Essential Tremor (ET) and Parkinson's disease (PD), since it is up-regulated in the cerebellum of these patients. Furthermore, a recent study demonstrated that adults with an extra copy of the LINGO1 gene also present with tremor, suggesting that increased levels of LINGO1 protein could be a causative factor in tremor.

Human LINGO1 Protein (LIN-HM201) Specifications/Details

Protein
 LINGO1
Synonyms
 LERN1; LRRN6A
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Cys42-Thr561
Molecular Weight
 The protein has a predicted MW of 84.98 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on Bis-Tris PAGE result.
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human LINGO1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human LINGO1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human LINGO1 is greater than 90% as determined by SEC-HPLC. The purity of Human LINGO1 is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...