Human HLA-A*02:01&B2M&LMP2 (CLGGLLTMV) Monomer Protein (MHC-HM411)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human HLA-A*02:01&B2M&LMP2 (CLGGLLTMV) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&LMP2 (CLGGLLTMV) Antibody, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and CLGGLLTMV peptide

Catalog: MHC-HM411

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Product Description Recombinant Human HLA-A*02:01&B2M&LMP2 (CLGGLLTMV) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and CLGGLLTMV peptide.

Product Background

The immunoproteasome, having been linked to neurodegenerative diseases and hematological cancers, has been shown to play an important role in MHC class I antigen presentation. The development of molecular probes that selectively inhibit the major catalytic subunit, LMP2, of the immunoproteasome,LMP2-rich cancer cells compared to LMP2-deficient cancer cells are more sensitive to growth inhibition by the LMP2-specific inhibitor, implicating an important role of LMP2 in regulating cell growth of malignant tumors that highly express LMP2.

Product Category

Recombinant Protein

Target

HLA-A*02:01&B2M&LMP2 (CLGGLLTMV) Monomer
Synonyms MHC; RMF; LMP2; LMP-2; Macropain chain 7; Proteasome chain 7; PSMB9; RING12
Accession
MHC Form Monomer
Allele HLA-A*02:01

Species

Human

Amino Acid Range

Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and CLGGLLTMV peptide
Molecular Weight The protein has a predicted MW of 50.3 kDa. Due to glycosylation, the protein migrates to 52-56 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human HLA-A*02:01&B2M&LMP2 (CLGGLLTMV) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&LMP2 (CLGGLLTMV) Antibody, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human HLA-A*02:01&B2M&LMP2 (CLGGLLTMV) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&LMP2 (CLGGLLTMV) Antibody, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA.
The purity of Human HLA-A*02:01&B2M&LMP2 (CLGGLLTMV) Monomer is greater than 95% as determined by SEC-HPLC.
Human HLA-A*02:01&B2M&LMP2 (CLGGLLTMV) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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