Human LRG1 Protein (LRG-HM101)
Human LRG1 Protein (LRG-HM101)
Human LRG1 Protein (LRG-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LRG1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LRG1 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA(QC Test). See testing image for detail.
  • Amino Acid Range: Val36-Gln347
Catalog #LRG-HM101
Regular price $300.00
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Product Description

Recombinant Human LRG1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val36-Gln347.

Product Background

Diabetic nephropathy (DN) is an important public health concern of increasing proportions and the leading cause of end-stage renal disease (ESRD) in diabetic patients. It is one of the most common long-term microvascular complications of diabetes mellitus that is characterized by proteinuria and glomerular structural changes. LRG1 is a novel pro-angiogenic factors involved in the abnormal angiogenesis and renal fibrosis in DN.

Product Category

Recombinant Protein / Antibody discovery

Protein

LRG1

Synonyms

Leucine-rich alpha-2-glycoprotein; LRG;LRG1

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Val36-Gln347

Molecular Weight

The protein has a predicted MW of 35.4 kDa. Due to glycosylation, the protein migrates to 48-52 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human LRG1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LRG1 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA(QC Test). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 24 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human LRG1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LRG1 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA(QC Test). Immobilized Human LRG1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LRG1 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA(QC Test).
Human LRG1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human LRG1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human LRG1 is greater than 95% as determined by SEC-HPLC. The purity of Human LRG1 is greater than 95% as determined by SEC-HPLC.

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