Human LRG1 Protein (LRG-HM101)
Human LRG1 Protein (LRG-HM101)
Human LRG1 Protein (LRG-HM101)
Human LRG1 Protein (LRG-HM101)
Human LRG1 Protein (LRG-HM101)
Human LRG1 Protein (LRG-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LRG1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LRG1 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Val36-Gln347
Catalog #LRG-HM101
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Product Description
 Recombinant Human LRG1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val36-Gln347.
Product Background
 Diabetic nephropathy (DN) is an important public health concern of increasing proportions and the leading cause of end-stage renal disease (ESRD) in diabetic patients. It is one of the most common long-term microvascular complications of diabetes mellitus that is characterized by proteinuria and glomerular structural changes. LRG1 is a novel pro-angiogenic factors involved in the abnormal angiogenesis and renal fibrosis in DN.
Product Category
 Recombinant Protein
Target
 LRG1
Synonyms
 Leucine-rich alpha-2-glycoprotein； LRG；LRG1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Val36-Gln347
Molecular Weight
 The protein has a predicted MW of 35.4 kDa. Due to glycosylation, the protein migrates to 48-52 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human LRG1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LRG1 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human LRG1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LRG1 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA.
Human LRG1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human LRG1 is greater than 95% as determined by SEC-HPLC.
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