Human LRG1 Protein (LRG-HM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LRG1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LRG1 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Val36-Gln347

Catalog: LRG-HM101

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Product Description Recombinant Human LRG1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val36-Gln347.

Product Background

Diabetic nephropathy (DN) is an important public health concern of increasing proportions and the leading cause of end-stage renal disease (ESRD) in diabetic patients. It is one of the most common long-term microvascular complications of diabetes mellitus that is characterized by proteinuria and glomerular structural changes. LRG1 is a novel pro-angiogenic factors involved in the abnormal angiogenesis and renal fibrosis in DN.

Product Category

Recombinant Protein

Target

LRG1
Synonyms Leucine-rich alpha-2-glycoprotein; LRG;LRG1

Gene/Protein ID

P02750

Species

Human

Exact Sequence

Val36-Gln347
Molecular Weight The protein has a predicted MW of 35.4 kDa. Due to glycosylation, the protein migrates to 48-52 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human LRG1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LRG1 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human LRG1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LRG1 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA.
The purity of Human LRG1 is greater than 95% as determined by SEC-HPLC.
Human LRG1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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