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Non-biotinylated Human LRRC15/LIB Protein, Ultra Low Endotoxin (LRR-HM415-UL) Summary
Product Description |
Recombinant Human LRRC15/LIB Protein is expressed from CHO with His tag and Avi tag at the C-Terminus.It contains Tyr22-Gly538. |
Product Background |
The leucine-rich repeat-containing protein 15 (LRRC15) is involved in cell–cell and cell–matrix interactions and came into focus as a promising anticancer target owing to its overexpression in mesenchymal-derived tumors such as sarcoma, glioblastoma, and melanoma and in cancer-associated fibroblasts in the microenvironment of breast, head and neck, lung, and pancreatic tumors. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Non-biotinylated Human LRRC15/LIB Protein, Ultra Low Endotoxin (LRR-HM415-UL) Specifications/Details
Protein |
LRRC15/LIB |
Synonyms |
LIB; LRRC15 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Tyr22-Gly538 |
Molecular Weight |
The protein has a predicted MW of 60.7 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Anti-LRRC15 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human LRRC15, His Tag with the EC50 of 12.0ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
CHO |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Anti-LRRC15 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human LRRC15, His Tag with the EC50 of 12.0ng/ml determined by ELISA. |
|
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Human LRRC15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human LRRC15 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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