Product Description |
Recombinant Human LY6E Protein is expressed from HEK293 with mFc (IgG1) tag at the C-terminus.It contains Leu21-Ser101. |
Product Background |
Lymphocyte antigen 6 complex, locus E (LY6E), also designated as stem cell antigen 2 (SCA2) and thymic shared antigen-1 (TSA-1), is a member of the lymphostromal cell membrane Ly6 superfamily. All Ly6 family members are low-molecular weight (10-12 kDa) proteins that display limited nucleotide and amino acid sequence homologies with one another, but commonly have a conserved cysteine-rich cell surface domain and are anchored to the cell surface via a glycosilphosphatidylinositol (GPI) moiety. |
Product Category |
Recombinant Protein |
Target |
LY6E |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu21-Ser101 |
Molecular Weight |
The protein has a predicted MW of 34.15 kDa. Due to glycosylation, the protein migrates to 40-48 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human LY6E, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-LY6E Antibody, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-mFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human LY6E, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-LY6E Antibody, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA. |
|
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Human LY6E on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human LY6E is greater than 95% as determined by SEC-HPLC. |
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