Human LY6E Protein (LYE-HM306)

  • Express System: HEK293
  • Product Tag: C-mFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LY6E, mFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LY6E Antibody, hFc Tag with the EC50 of 18.3ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Leu21-Ser101

Catalog: LYE-HM306

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Product Description Recombinant Human LY6E Protein is expressed from HEK293 with mFc tag at the C-terminus.It contains Leu21-Ser101.

Product Background

Lymphocyte antigen 6 complex, locus E (LY6E), also designated as stem cell antigen 2 (SCA2) and thymic shared antigen-1 (TSA-1), is a member of the lymphostromal cell membrane Ly6 superfamily. All Ly6 family members are low-molecular weight (10-12 kDa) proteins that display limited nucleotide and amino acid sequence homologies with one another, but commonly have a conserved cysteine-rich cell surface domain and are anchored to the cell surface via a glycosilphosphatidylinositol (GPI) moiety.

Product Category

Recombinant Protein

Target

LY6E

Gene/Protein ID

Q16553

Species

Human

Exact Sequence

Leu21-Ser101
Molecular Weight The protein has a predicted MW of 34.15 kDa. Due to glycosylation, the protein migrates to 40-48 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human LY6E, mFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LY6E Antibody, hFc Tag with the EC50 of 18.3ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-mFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human LY6E, mFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LY6E Antibody, hFc Tag with the EC50 of 18.3ng/ml determined by ELISA.
The purity of Human LY6E is greater than 95% as determined by SEC-HPLC.
Human LY6E on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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