Human LY6E Protein (LYE-HM306)
Human LY6E Protein (LYE-HM306)
Human LY6E Protein (LYE-HM306)
Human LY6E Protein (LYE-HM306)
Human LY6E Protein (LYE-HM306)
Human LY6E Protein (LYE-HM306)
  • Express System: HEK293
  • Product Tag: C-mFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LY6E, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-LY6E Antibody, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Leu21-Ser101
Catalog #LYE-HM306
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Product Description
 Recombinant Human LY6E Protein is expressed from HEK293 with mFc (IgG1) tag at the C-terminus.It contains Leu21-Ser101.
Product Background
 Lymphocyte antigen 6 complex, locus E (LY6E), also designated as stem cell antigen 2 (SCA2) and thymic shared antigen-1 (TSA-1), is a member of the lymphostromal cell membrane Ly6 superfamily. All Ly6 family members are low-molecular weight (10-12 kDa) proteins that display limited nucleotide and amino acid sequence homologies with one another, but commonly have a conserved cysteine-rich cell surface domain and are anchored to the cell surface via a glycosilphosphatidylinositol (GPI) moiety.
Product Category
 Recombinant Protein
Target
 LY6E
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Leu21-Ser101
Molecular Weight
 The protein has a predicted MW of 34.15 kDa. Due to glycosylation, the protein migrates to 40-48 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human LY6E, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-LY6E Antibody, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-mFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human LY6E, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-LY6E Antibody, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA.
Human LY6E on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human LY6E is greater than 95% as determined by SEC-HPLC.
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