Human M-CSF R/CSF1R/CD115 Protein (CSF-HM21R)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human M-CSF, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ile20-Glu512

Catalog: CSF-HM21R

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Product Description	Recombinant Human M-CSF R/CSF1R/CD115 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ile20-Glu512.
Product Category	Recombinant Protein

Target	M-CSF R/CSF1R/CD115
Synonyms	CD115; CSF1R; CSF-1-R; CSFR; FMSFIM2; M-CSF R; MCSFR; M-CSFR; C-FMS; FIM2; FMS; HDLS
Gene/Protein ID	P07333-1
Species	Human
Exact Sequence	Ile20-Glu512
Molecular Weight	The protein has a predicted MW of 81.3 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human M-CSF, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human M-CSF, His Tag captured on CM5 Chip via Anti-his Antibody can bind Human M-CSF R, hFc Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).
	Immobilized Human M-CSF, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA (QC Test).
	The purity of Human M-CSF R is greater than 95% as determined by SEC-HPLC.
	Human M-CSF R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.