Human M-CSF R/CSF1R/CD115 Protein (CSF-HM21R)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human M-CSF at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 12.4ng/ml determined by ELISA. The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ile20-Glu512

Catalog: CSF-HM21R

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Product Description Recombinant Human M-CSF R/CSF1R/CD115 Protein is expressed from Expi293 with hFc tag at the C-terminal.; It contains Ile20-Glu512.
Product Category Recombinant Protein
Target M-CSF R/CSF1R/CD115
Synonyms CD115; CSF1R; CSF-1-R; CSFR; FMSFIM2; M-CSF R; MCSFR; M-CSFR; C-FMS; FIM2; FMS; HDLS
Gene/Protein ID P07333-1
Species Human
Exact Sequence Ile20-Glu512
Molecular Weight The protein has a predicted MW of 81.3 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human M-CSF at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 12.4ng/ml determined by ELISA. The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human M-CSF, His Tag captured on CM5 Chip via Anti-his Antibody can bind Human M-CSF R, hFc Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).
Immobilized Human M-CSF at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 12.4ng/ml determined by ELISA.
The purity of Human M-CSF R is greater than 95% as determined by SEC-HPLC.
Human M-CSF R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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