Human MADCAM1 Protein (MCM-HM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human MADCAM1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gln19-Gln317

Catalog: MCM-HM101

Order now get it on

Bulk Quote | Test Sample

Product Description	Recombinant Human MADCAM1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln19-Gln317.
Product Background	Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) contributes to the recruitment of donor T cells into the mucosal tissues of the recipient after allogeneic hematopoietic stem cell transplantation (aHSCT). The aim of our study was to determine whether selected single nucleotide polymorphisms (SNPs) of the MADCAM1 gene are associated with development of serious complications after aHSCT.
Product Category	Recombinant Protein

Target	MADCAM1
Synonyms	MAdCAM-1; MADCAM1; MACAM1
Gene/Protein ID	Q13477-1
Species	Human
Exact Sequence	Gln19-Gln317
Molecular Weight	The protein has a predicted MW of 32.5 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human MADCAM1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human MADCAM1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA.
	The purity of Human MADCAM1 is greater than 95% as determined by SEC-HPLC.
	Human MADCAM1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.