Name: Human MADCAM1 Protein (MCM-HM101)
Brand: KactusBio
SKU: MCM-HM101-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human MADCAM1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test).
Amino Acid Range: Gln19-Gln317

Catalog #MCM-HM101

Size

100UG

500UG

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Human MADCAM1 Protein (MCM-HM101) Summary

Product Description	Recombinant Human MADCAM1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln19-Gln317.
Product Background	Mucosal addressin cell adhesion molecule–1 (MAdCAM-1) is an endothelial cell marker that is recognized by its ligands on lymphocytes: L-selectin and α4β7 integrin. MAdCAM-1 is highly expressed on intestinal endothelium and has, recently, been shown to play an important role in the development of acute GVHD in animal model.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human MADCAM1 Protein (MCM-HM101) Specifications/Details

Protein	MADCAM1
Synonyms	MAdCAM-1; MADCAM1; MACAM1
Accession	Q13477-1
Species	Human
Biotinylated	no
Amino Acid Range	Gln19-Gln317
Molecular Weight	The protein has a predicted MW of 32.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human MADCAM1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test).
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human MADCAM1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test).
	Human MADCAM1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Anti-MADCAM1 Antibody, hFc Tag on ProA-Biosensor can bind Human MADCAM1, His Tag with an affinity constant of 9.41 nM as determined in BLI assay .

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