Human MADCAM1 Protein (MCM-HM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human MADCAM1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln19-Gln317

Catalog: MCM-HM101

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Product Description Recombinant Human MADCAM1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln19-Gln317.

Product Background

Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) contributes to the recruitment of donor T cells into the mucosal tissues of the recipient after allogeneic hematopoietic stem cell transplantation (aHSCT). The aim of our study was to determine whether selected single nucleotide polymorphisms (SNPs) of the MADCAM1 gene are associated with development of serious complications after aHSCT.

Product Category

Recombinant Protein

Target

MADCAM1
Synonyms MAdCAM-1; MADCAM1; MACAM1
Accession

Species

Human

Exact Sequence

Gln19-Gln317
Molecular Weight The protein has a predicted MW of 32.5 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human MADCAM1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human MADCAM1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA.
The purity of Human MADCAM1 is greater than 95% as determined by SEC-HPLC.
Human MADCAM1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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