• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human MADCAM1, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA (QC Test). The affinity constant of 45.41 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Gln19-Gln317
Catalog #MCM-HM201-UL
Price
Regular price $32700
Regular price Sale price $32700
/
Size
Avantor VWR Fisher Scientific

Order by 4pm ET for delivery on:

Human MADCAM1 Protein, Ultra Low Endotoxin (MCM-HM201-UL) Summary

Product Description

 Recombinant Human MADCAM1 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gln19-Gln317.

Product Background

 Mucosal addressin cell adhesion molecule–1 (MAdCAM-1) is an endothelial cell marker that is recognized by its ligands on lymphocytes: L-selectin and α4β7 integrin. MAdCAM-1 is highly expressed on intestinal endothelium and has, recently, been shown to play an important role in the development of acute GVHD in animal model.

Human MADCAM1 Protein, Ultra Low Endotoxin (MCM-HM201-UL) Specifications/Details

Protein
 MADCAM1
Synonyms
 MAdCAM-1; MADCAM1; MACAM1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gln19-Gln317
Molecular Weight
 The protein has a predicted MW of 58.17 kDa. Due to glycosylation, the protein migrates to 75-95 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human MADCAM1, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA (QC Test). The affinity constant of 45.41 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human MADCAM1, hFc Tag captured on CM5 Chip via Protein A can bind Human ITGA4&ITGB7, His Tag (Cat. ITG-HM147) with an affinity constant of 45.41 nM as determined in SPR assay (Biacore S200). Human MADCAM1, hFc Tag captured on CM5 Chip via Protein A can bind Human ITGA4&ITGB7, His Tag (Cat. ITG-HM147) with an affinity constant of 45.41 nM as determined in SPR assay (Biacore S200).
Immobilized Human MADCAM1, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA (QC Test). Immobilized Human MADCAM1, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA (QC Test).
Immobilized Human ITGA4&ITGB7, His Tag (Cat. ITG-HM147) at 5ug/ml (100ul/well) on the plate. Dose response curve for Human MADCAM1, hFc Tag with the EC50 of 9.2ng/ml determined by ELISA. Immobilized Human ITGA4&ITGB7, His Tag (Cat. ITG-HM147) at 5ug/ml (100ul/well) on the plate. Dose response curve for Human MADCAM1, hFc Tag with the EC50 of 9.2ng/ml determined by ELISA.
Human MADCAM1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human MADCAM1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human MADCAM1 is greater than 90% as determined by SEC-HPLC. The purity of Human MADCAM1 is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...