Name: Human MADCAM1 Protein, Ultra Low Endotoxin (MCM-HM201-UL)
Brand: KactusBio
SKU: MCM-HM201-UL-100UG
Price: 327.0 USD
Availability: InStock

Human MADCAM1 Protein, Ultra Low Endotoxin (MCM-HM201-UL)

Immobilized Human MADCAM1, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA (QC Test).

Immobilized Human ITGA4&ITGB7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human MADCAM1, hFc Tag with the EC50 of 5.5ug/ml determined by ELISA.

Human MADCAM1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human MADCAM1 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human MADCAM1, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Gln19-Gln317

Catalog #MCM-HM201-UL

Size

100UG

500UG

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Product Description	Recombinant Human MADCAM1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln19-Gln317.
Product Background	Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) contributes to the recruitment of donor T cells into the mucosal tissues of the recipient after allogeneic hematopoietic stem cell transplantation (aHSCT). The aim of our study was to determine whether selected single nucleotide polymorphisms (SNPs) of the MADCAM1 gene are associated with development of serious complications after aHSCT.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	MADCAM1
Synonyms	MAdCAM-1; MADCAM1; MACAM1
Accession	Q13477-1
Species	Human
Biotinylated	no
Amino Acid Range	Gln19-Gln317
Molecular Weight	The protein has a predicted MW of 58.17 kDa. Due to glycosylation, the protein migrates to 75-95 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human MADCAM1, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.01 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human MADCAM1, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA (QC Test).
	Immobilized Human ITGA4&ITGB7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human MADCAM1, hFc Tag with the EC50 of 5.5ug/ml determined by ELISA.
	Human MADCAM1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human MADCAM1 is greater than 95% as determined by SEC-HPLC.