Name: Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer Protein (MHC-HM445)
Brand: KactusBio
SKU: MHC-HM445-100UG
Price: 310.0 USD
Availability: InStock

Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer Protein (MHC-HM445)

Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer, His Tag captured on CM5 Chip via Anti-His Antibody can bind HLA-A*02:01&B2M&MAGE-A1 TCR with an affinity constant of 116.2 nM as determined in SPR assay (Biacore T200).

Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer -on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 116.2 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KVLEYVIKV peptide

Catalog #MHC-HM445

Size

100UG

500UG

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Product Description	Recombinant Human HLA-A02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer-Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A02:01), Ile21-Met119(B2M) and KVLEYVIKV peptide.
Product Background	MAGE-A1 belongs to the chromosome X-clustered genes of cancer-testis antigen family and is normally expressed in the human germ line but is also overexpressed in various tumors.
Product Category	Recombinant Protein / Antibody discovery / MHC

Protein	HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer
Synonyms	MAGE-A1; MAGE A1; CT1.1; MAGE-1 antigen; MAGE-1; MZ2-E; MAGE1; MAGE1A
MHC Form	Monomer
Allele	HLA-A*02:01
Accession	A0A140T913(HLA-A*02:01)&P61769(B2M)&KVLEYVIKV
Species	Human
Biotinylated	no
Amino Acid Range	Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KVLEYVIKV peptide
Molecular Weight	The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 53-65 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 116.2 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human HLA-A02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer, His Tag captured on CM5 Chip via Anti-His Antibody can bind HLA-A02:01&B2M&MAGE-A1 TCR with an affinity constant of 116.2 nM as determined in SPR assay (Biacore T200).
	Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer -on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer is greater than 95% as determined by SEC-HPLC.