HPLC chromatogram showing a sharp single peak at 7.160 minutes, indicating high purity of the recombinant protein sample
Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Tetramer Protein (MHC-HM445T)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KVLEYVIKV peptide
Catalog #MHC-HM445T
Regular price $825.00
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Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Tetramer Protein (MHC-HM445T) Summary

Product Description

Recombinant Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KVLEYVIKV peptide.

Product Background

MAGE-A1 belongs to the chromosome X-clustered genes of cancer-testis antigen family and is normally expressed in the human germ line but is also overexpressed in various tumors.

Product Category

Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs

HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Specifications

Protein
HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV)
Synonyms
MAGE-A1; MAGE A1; CT1.1; MAGE-1 antigen; MAGE-1; MZ2-E; MAGE1; MAGE1A

MHC Form

Tetramer

Allele

HLA-A*02:01
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KVLEYVIKV peptide
Molecular Weight
The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.

Product Tag

C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%. Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%.
The purity of Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Tetramer is greater than 95% as determined by SEC-HPLC. The purity of Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Tetramer is greater than 95% as determined by SEC-HPLC.
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