Name: Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Tetramer Protein (MHC-HM427T)
Brand: KactusBio
SKU: MHC-HM427T-100UG
Price: 750.0 USD
Availability: InStock

Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Tetramer Protein (MHC-HM427T)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Amino Acid Range: Gly25-Thr305(HLA-A*01:01),Ile21-Met119(B2M)and EVDPIGHLY peptide

Catalog #MHC-HM427T

Size

100UG

500UG

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Product Description	Recombinant Human MAGE-A3(HLA-A01:01) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A01:01),Ile21-Met119(B2M) and EVDPIGHLY peptide.
Product Background	Melanoma antigen gene A3 (MAGE-A3) is one of the most immunogenic cancer testis antigens and is common in various types of cancers. MAGE-A3 can be considered as a predictor for poor prognosis and an option for vaccine immunotherapy in patients with PCa.
Product Category	Recombinant Protein

Target	HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Tetramer
MHC Form	Tetramer
Allele	HLA-A*01:01
Accession	Q5SUL5(HLA-A*01:01)&P61769(B2M)&EVDPIGHLY
Species	Human
Biotinylated	no
Amino Acid Range	Gly25-Thr305(HLA-A*01:01),Ile21-Met119(B2M)and EVDPIGHLY peptide
Molecular Weight	The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Tetramer on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%.
	The purity of Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Tetramer is greater than 95% as determined by SEC-HPLC.