Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Tetramer Protein (MHC-HM427T)
Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Tetramer Protein (MHC-HM427T)
Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Tetramer Protein (MHC-HM427T)
Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Tetramer Protein (MHC-HM427T)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gly25-Thr305(HLA-A*01:01),Ile21-Met119(B2M)and EVDPIGHLY peptide
Catalog #MHC-HM427T
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Product Description
 Recombinant Human MAGE-A3(HLA-A*01:01) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*01:01),Ile21-Met119(B2M) and EVDPIGHLY peptide.
Product Background
 Melanoma antigen gene A3 (MAGE-A3) is one of the most immunogenic cancer testis antigens and is common in various types of cancers. MAGE-A3 can be considered as a predictor for poor prognosis and an option for vaccine immunotherapy in patients with PCa.
Product Category
 Recombinant Protein
Target
 HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Tetramer
MHC Form
 Tetramer
Allele
 HLA-A*01:01
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly25-Thr305(HLA-A*01:01),Ile21-Met119(B2M)and EVDPIGHLY peptide
Molecular Weight
 The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Tetramer on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%.
The purity of Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Tetramer is greater than 95% as determined by SEC-HPLC.
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