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Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Tetramer Protein (MHC-HM427T) Summary
Product Description |
Recombinant Human MAGE-A3(HLA-A*01:01) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*01:01), Ile21-Met119(B2M) and EVDPIGHLY peptide. |
Product Background |
Melanoma antigen gene A3 (MAGE-A3) is one of the most immunogenic cancer testis antigens and is common in various types of cancers. MAGE-A3 can be considered as a predictor for poor prognosis and an option for vaccine immunotherapy in patients with PCa. |
Product Category |
Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs |
HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Specifications
Protein |
HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) |
Synonyms |
Melanoma-associated antigen 3; MZ2-D; MZ2D; MZ2-D; CT1.3; MAGE-3 |
MHC Form |
Tetramer |
Allele |
HLA-A*01:01 |
Accession |
Q5SUL5(HLA-A*01:01)&P61769(B2M)&EVDPIGHLY |
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly25-Thr305(HLA-A*01:01), Ile21-Met119(B2M) and EVDPIGHLY peptide |
Molecular Weight |
The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result. |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%. |
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The purity of Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Tetramer is greater than 95% as determined by SEC-HPLC. |
Additional Information
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