Human MAGE-A4 (HLA-A*02:01) Protein (MHC-HM401)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human MAGE-A4 (HLA-A*02:01) , His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-MAGE-A4 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 12.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and GVYDGREHTV peptide

Catalog: MHC-HM401

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Product Description Recombinant Human MAGE-A4 (HLA-A*02:01) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and GVYDGREHTV peptide.
Product Category Recombinant Protein
Target MAGE-A4 (HLA-A*02:01)
Synonyms HLA0201; MHC I; MAGE-A4; CT1.4; MAGE4A; MAGE4B; MAGE-X2; member 4
Gene/Protein ID A0A140T913(HLA-A*02:01)&P61769(B2M)&GVYDGREHTV
Species Human
Exact Sequence Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and GVYDGREHTV peptide
Molecular Weight The protein has a predicted MW of 50.5 kDa. Due to glycosylation,the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human MAGE-A4 (HLA-A*02:01) , His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-MAGE-A4 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 12.7ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human MAGE-A4 (HLA-A*02:01) , His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-MAGE-A4 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 12.7ng/ml determined by ELISA.
The purity of Human MAGE-A4 (HLA-A*02:01) is greater than 95% as determined by SEC-HPLC.
Human MAGE-A4 (HLA-A*02:01) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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