Name: Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer Protein (MHC-HM401T)
Brand: KactusBio
SKU: MHC-HM401T-100UG
Price: 825.0 USD
Availability: InStock

Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer Protein (MHC-HM401T)

Anti-HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer, His Tag with an affinity constant of 8.49pM as determined in SPR assay (Biacore T200).

Immobilized Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).

Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%.

The purity of Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). The affinity constant of 8.49pM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and GVYDGREHTV peptide

Catalog #MHC-HM401T

Size

100UG

500UG

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Product Description	Recombinant Human HLA-A02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A02:01), Ile21-Met119(B2M) and GVYDGREHTV peptide.
Product Background	Melanoma-associated antigen 4 is a protein that in humans is encoded by the MAGEA4 gene.The MAGE- A4 antigen is among the most commonly expressed cancer testis antigens. The Human HLA-A0201 MAGE-A4 (GVYDGREHTV) complex Protein is a complex of HLA-A0201 of -the MHC Class I, B2M and GVYDGREHTV peptide of -the MAGE-A4.
Product Category	Recombinant Protein / Antibody discovery / MHC

Protein	HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer
Synonyms	HLA0201; MHC I; MAGE-A4; CT1.4; MAGE4A; MAGE4B; MAGE-X2; member 4
MHC Form	Tetramer
Allele	HLA-A*02:01
Accession	A0A140T913(HLA-A*02:01)&P61769(B2M)&GVYDGREHTV
Species	Human
Biotinylated	no
Amino Acid Range	Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and GVYDGREHTV peptide
Molecular Weight	The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.
Activity	Immobilized Human HLA-A02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-HLA-A02:01&B2M&MAGE-A4 (GVYDGREHTV) Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). The affinity constant of 8.49pM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Anti-HLA-A02:01&B2M&MAGE-A4 (GVYDGREHTV) Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human HLA-A02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer, His Tag with an affinity constant of 8.49pM as determined in SPR assay (Biacore T200).
	Immobilized Human HLA-A02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-HLA-A02:01&B2M&MAGE-A4 (GVYDGREHTV) Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).
	Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%.
	The purity of Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Tetramer is greater than 95% as determined by SEC-HPLC.