Human MANSC1 Protein (MAN-HM1C1)

Immobilized Human MANSC1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MANSC1 Antibody, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA.

Human MANSC1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human MANSC1 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human MANSC1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MANSC1 Antibody, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA.
Amino Acid Range: Gln27-Leu385

Catalog #MAN-HM1C1

Price

$297⁰⁰

Size

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Human MANSC1 Protein (MAN-HM1C1) Summary

Product Description

Recombinant Human MANSC1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln27-Leu385.

Product Background

MANSC1 contains 1 MANSC domain. MANSC is a seven-cysteine-containing domain present in animal membrane and extracellular proteins. MANSC (motif at N terminus with seven cysteines) is a novel domain with a well-conserved seven-cysteine motif that is present at the N terminus of membrane and extracellular proteins, including low-density lipoprotein receptor-related protein 11 (LRP-11), hepatocyte growth factor activator inhibitor 1 (HAI-1) and some uncharacterized proteins encoded by multicellular animals from Mollusca to Chordata. The MANSC domain in HAI-2 might function through binding with hepatocyte growth factor activator and matriptase[1].

Human MANSC1 Protein (MAN-HM1C1) Specifications/Details

Protein	MANSC1
Synonyms	MANSC1; LOH12CR3; 9130403P13Rik
Accession	Q9H8J5
Species	Human
Biotinylated	no
Amino Acid Range	Gln27-Leu385
Molecular Weight	The protein has a predicted MW of 39.9 kDa. Due to glycosylation, the protein migrates to 113-116 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human MANSC1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MANSC1 Antibody, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human MANSC1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MANSC1 Antibody, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA.
	Human MANSC1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human MANSC1 is greater than 95% as determined by SEC-HPLC.