Human MANSC1 Protein (MAN-HM1C1)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human MANSC1, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-MANSC1 Antibody, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gln27-Leu385

Catalog: MAN-HM1C1

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Product Description	Recombinant Human MANSC1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln27-Leu385.
Product Background	MANSC1 contains 1 MANSC domain. MANSC is a seven-cysteine-containing domain present in animal membrane and extracellular proteins. MANSC (motif at N terminus with seven cysteines) is a novel domain with a well-conserved seven-cysteine motif that is present at the N terminus of membrane and extracellular proteins, including low-density lipoprotein receptor-related protein 11 (LRP-11), hepatocyte growth factor activator inhibitor 1 (HAI-1) and some uncharacterized proteins encoded by multicellular animals from Mollusca to Chordata. The MANSC domain in HAI-2 might function through binding with hepatocyte growth factor activator and matriptase[1].
Product Category	Recombinant Protein

Target	MANSC1
Synonyms	MANSC1; LOH12CR3; 9130403P13Rik
Gene/Protein ID	Q9H8J5
Species	Human
Exact Sequence	Gln27-Leu385
Molecular Weight	The protein has a predicted MW of 39.9 kDa. Due to glycosylation, the protein migrates to 113-116 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human MANSC1, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-MANSC1 Antibody, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human MANSC1, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-MANSC1 Antibody, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA.
	The purity of Human MANSC1 is greater than 95% as determined by SEC-HPLC.
	Human MANSC1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.