Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer Protein (MHC-HM435)
Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer Protein (MHC-HM435)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ELAGIGILTV peptide
Catalog #MHC-HM435
Regular price $341.00
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Product Description

Recombinant Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ELAGIGILTV peptide.

Product Background

Melanoma antigen recognized by T cell-1 (Mart-1), one of the melanosome-specific proteins, also recognized by cytotoxicity T lymphocytes as a marker. Mart-1 is considered to play a critical role in the immunotherapy for melanoma.

Product Category

Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs

Protein

HLA-A*02:01&B2M&MART-1 (ELAGIGILTV)

Synonyms

MART1; LB39-AA; SK29-AA; Melan-A; MLANA

MHC Form

Monomer

Allele

HLA-A*02:01

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ELAGIGILTV peptide

Molecular Weight

The protein has a predicted MW of 50.40 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer is greater than 95% as determined by SEC-HPLC. The purity of Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer is greater than 95% as determined by SEC-HPLC.

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