Product Description |
Recombinant Human MASP2 Protein is expressed from E.coli with His tag at the C-Terminus.It contains Gly288-Phe686. |
Product Background |
The pathogenesis of severe acute respiratory disease syndrome (SARS) is not fully understood. One case-control study has reported an association between susceptibility to SARS and mannan-binding lectin (MBL) in China. As the downstream protein of MBL, variants of the MBL-associated serine protease-2 (MASP2) gene may be associated with SARS coronavirus (SARS-CoV) infection in the same population. |
Product Category |
Recombinant Protein |
Target |
MASP2 |
Synonyms |
MAP19; MASP-2; MASP1P1; sMap |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly288-Phe686 |
Molecular Weight |
The protein has a predicted MW of 46.9 kDa. Due to autocatalytic cleavage, the protein migrates to 18 kDa&25-30 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human MASP2, His Tag at 0.5 ug/ml (100 ul/Well). Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 5.0 ng/ml determined by ELISA (QC Test). The affinity constant of 3.08 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
E.coli |
Purity |
>95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 20 mM Tris, 200 mM NaCl (pH 9.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Anti-MASP2 Antibody captured on CM5 Chip via Protein A can bind Human MASP2, His Tag with an affinity constant of 3.08 nM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Human MASP2, His Tag at 0.5 ug/ml (100 ul/Well). Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 5.0 ng/ml determined by ELISA (QC Test). |
|
|
Human MASP2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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