Human MASP2 Protein (MSP-HE102)
  • Express System: E.coli
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human MASP2, His Tag at 0.5ug/ml (100ul/Well). Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test). The affinity constant of 3.08 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly288-Phe686
Catalog #MSP-HE102
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Product Description
 Recombinant Human MASP2 Protein is expressed from E.coli with His tag at the C-Terminus.It contains Gly288-Phe686.
Product Background
 The pathogenesis of severe acute respiratory disease syndrome (SARS) is not fully understood. One case-control study has reported an association between susceptibility to SARS and mannan-binding lectin (MBL) in China. As the downstream protein of MBL, variants of the MBL-associated serine protease-2 (MASP2) gene may be associated with SARS coronavirus (SARS-CoV) infection in the same population.
Product Category
 Recombinant Protein
Target
 MASP2
Synonyms
 MAP19; MASP-2; MASP1P1; sMap
Accession
Species
 Human
Amino Acid Range
 Gly288-Phe686
Molecular Weight
 The protein has a predicted MW of 46.9 kDa. Due to autocatalytic cleavage, the protein migrates to 18 kDa&25-30 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human MASP2, His Tag at 0.5ug/ml (100ul/Well). Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test). The affinity constant of 3.08 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in 20mM Tris, 200mM NaCl (pH 9.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Anti-MASP2 Antibody captured on CM5 Chip via Protein A can bind Human MASP2, His Tag with an affinity constant of 3.08 nM as determined in SPR assay (Biacore T200).
Immobilized Human MASP2, His Tag at 0.5ug/ml (100ul/Well). Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test).
Human MASP2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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