Name: Human MASP2 Protein (MSP-HE102)
Brand: KactusBio
SKU: MSP-HE102-100UG
Price: 445.5 USD
Availability: InStock

Express System: E.coli
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human MASP2, His Tag at 0.5 ug/ml (100 ul/Well). Dose response curve for Narsoplimab, hFc Tag with the EC50 of 4.9 ng/ml determined by ELISA. The affinity constant of 3.08 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Gly288-Phe686
Other Available Proteins:

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Catalog #MSP-HE102

Size

100UG

500UG

Order by 4pm ET for delivery on:

Human MASP2 Protein (MSP-HE102) Summary

Product Description	Recombinant Human MASP2 Protein is expressed from E.coli with His tag at the C-Terminus.It contains Gly288-Phe686.
Product Background	Te MASP2 gene is located on the chromosome 1p36.23–31 and encodes two proteins, MASP-2 and Map198. Both the levels and function of MASP-2 are modulated by the single-nucleotide polymorphisms (SNPs) of the MASP2 gene. Te level of MASP-2 in plasma plays a dual role in the infection and progression of infectious diseases. Te defciency of MASP-2 in plasma has been associated with the susceptibility of HIV. By contrast, the overproduction of MASP-2 may increase the infammatory efect.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human MASP2 Protein (MSP-HE102) Specifications/Details

Protein	MASP2
Synonyms	MAP19; MASP-2; MASP1P1; sMap
Accession	O00187-1
Species	Human
Biotinylated	no
Amino Acid Range	Gly288-Phe686
Molecular Weight	The protein has a predicted MW of 46.9 kDa. Due to autocatalytic cleavage, the protein migrates to 18 kDa&25-30 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human MASP2, His Tag at 0.5 ug/ml (100 ul/Well). Dose response curve for Narsoplimab, hFc Tag with the EC50 of 4.9 ng/ml determined by ELISA. The affinity constant of 3.08 nM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 20 mM Tris, 200 mM NaCl (pH 9.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Narsoplimab captured on CM5 Chip via Protein A can bind Human MASP2, His Tag with an affinity constant of 3.08 nM as determined in SPR assay (Biacore T200).
	Immobilized Human MASP2, His Tag at 0.5 ug/ml (100 ul/Well). Dose response curve for Narsoplimab, hFc Tag with the EC50 of 4.9 ng/ml determined by ELISA.
	Human MASP2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Narsoplimab on ProA-Biosensor can bind Human MASP2, His Tag with an affinity constant of 10.00 nM as determined in BLI assay .
	The purity of Human MASP2 is greater than 95% as determined by SEC-HPLC.

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