Human MASP2 Protein (MSP-HE102)

  • Express System: E.coli
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human MASP2, His Tag at 0.2μg/ml (100μl/Well). Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). The affinity constant of 3.08 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gly288-Phe686

Catalog: MSP-HE102

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Product Description Recombinant Human MASP2 Protein is expressed from E.coli with His tag at the C-terminal.It contains Gly288-Phe686.
Product Category Recombinant Protein
Target MASP2
Synonyms MAP19; MASP-2; MASP1P1; sMap
Gene/Protein ID O00187-1
Species Human
Exact Sequence Gly288-Phe686
Molecular Weight The protein has a predicted MW of 46.9 kDa. Due to autocatalytic cleavage, the protein migrates to 18 kDa&25-30 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human MASP2, His Tag at 0.2μg/ml (100μl/Well). Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). The affinity constant of 3.08 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System E.coli
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in 20mM Tris, 200mM NaCl (pH 9.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Anti-MASP2 Antibody captured on CM5 Chip via Protein A can bind Human MASP2, His Tag with an affinity constant of 3.08 nM as determined in SPR assay (Biacore T200).
Immobilized Human MASP2, His Tag at 0.2μg/ml (100μl/Well). Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test).
Human MASP2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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