• Express System: E.coli
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human MASP2, His Tag at 0.5 ug/ml (100 ul/Well). Dose response curve for Narsoplimab, hFc Tag with the EC50 of 4.9 ng/ml determined by ELISA. The affinity constant of 3.08 nM as determined in SPR assay (Biacore T200). See testing image for detail.Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5'Dithio-bis. The specific activity is >2800 pmol/min/µg.
  • Amino Acid Range: Gly288-Phe686
Catalog #MSP-HE102
Price
Regular price $44550
Regular price Sale price $44550
/
Size
Avantor VWR Fisher Scientific

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Human MASP2 Protein (MSP-HE102) Summary

Product Description

 Recombinant Human MASP2 Protein is expressed from E.coli with His tag at the C-Terminus.It contains Gly288-Phe686.

Product Background

 Te MASP2 gene is located on the chromosome 1p36.23–31 and encodes two proteins, MASP-2 and Map198. Both the levels and function of MASP-2 are modulated by the single-nucleotide polymorphisms (SNPs) of the MASP2 gene. Te level of MASP-2 in plasma plays a dual role in the infection and progression of infectious diseases. Te defciency of MASP-2 in plasma has been associated with the susceptibility of HIV. By contrast, the overproduction of MASP-2 may increase the infammatory efect.

Human MASP2 Protein (MSP-HE102) Specifications/Details

Protein
 MASP2
Synonyms
 MAP19; MASP-2; MASP1P1; sMap;MASP2
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly288-Phe686
Molecular Weight
 The protein has a predicted MW of 46.9 kDa. Due to autocatalytic cleavage, the protein migrates to 18 kDa&25-30 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human MASP2, His Tag at 0.5 ug/ml (100 ul/Well). Dose response curve for Narsoplimab, hFc Tag with the EC50 of 4.9 ng/ml determined by ELISA. The affinity constant of 3.08 nM as determined in SPR assay (Biacore T200). See testing image for detail.Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5'Dithio-bis. The specific activity is >2800 pmol/min/µg.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in 20 mM Tris, 200 mM NaCl (pH 9.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Narsoplimab captured on CM5 Chip via Protein A can bind Human MASP2, His Tag with an affinity constant of 3.08 nM as determined in SPR assay (Biacore T200). Narsoplimab captured on CM5 Chip via Protein A can bind Human MASP2, His Tag with an affinity constant of 3.08 nM as determined in SPR assay (Biacore T200).
Immobilized Human MASP2, His Tag at 0.5 ug/ml (100 ul/Well). Dose response curve for Narsoplimab, hFc Tag with the EC50 of 4.9 ng/ml determined by ELISA. Immobilized Human MASP2, His Tag at 0.5 ug/ml (100 ul/Well). Dose response curve for Narsoplimab, hFc Tag with the EC50 of 4.9 ng/ml determined by ELISA.
Human MASP2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human MASP2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Narsoplimab on ProA-Biosensor can bind Human MASP2, His Tag with an affinity constant of 10.00 nM as determined in BLI assay. Loaded Narsoplimab on ProA-Biosensor can bind Human MASP2, His Tag with an affinity constant of 10.00 nM as determined in BLI assay.
The purity of Human MASP2 is greater than 95% as determined by SEC-HPLC. The purity of Human MASP2 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...