Human MASP2 Protein (MSP-HE102)
Catalog: MSP-HE102
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| Product Description | Recombinant Human MASP2 Protein is expressed from E.coli with His tag at the C-terminal.It contains Gly288-Phe686. |
| Product Category | Recombinant Protein |
| Target | MASP2 |
| Synonyms | MAP19; MASP-2; MASP1P1; sMap |
| Gene/Protein ID | O00187-1 |
| Species | Human |
| Exact Sequence | Gly288-Phe686 |
| Molecular Weight | The protein has a predicted MW of 46.9 kDa. Due to Autocatalytic cleavage, the protein migrates to 18 kDa&25-30 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human MASP2, His Tag at 0.2μg/ml (100μl/Well). Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA. The affinity constant of 3.08 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His |
| Express System | E.coli |
| Purity | > 95% as determined by Tris-Bis PAGE |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Liquid |
| Shipping | Shipped with blue ice. |
| Formulation | Supplied as 0.22μm filtered solution in 20mM Tris, 200mM NaCl (pH 9.0). Please dilute to the desired concentration according to the concentration of the solution shown on the product label. |
| Stability And Storage | Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles. |
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Anti-MASP2 Antibody captured on CM5 Chip via Protein A can bind Human MASP2, His Tag with an affinity constant of 3.08 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human MASP2, His Tag at 0.2μg/ml (100μl/Well). Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA. |
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Human MASP2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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