Human MASP2 Protein (MSP-HE102)

  • Express System: E.coli
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human MASP2, His Tag at 0.5ug/ml (100ul/Well). Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test). The affinity constant of 3.08 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly288-Phe686

Catalog: MSP-HE102

$405.00
Size:

Order now get it on

Product Description Recombinant Human MASP2 Protein is expressed from E.coli with His tag at the C-Terminus.It contains Gly288-Phe686.

Product Background

The pathogenesis of severe acute respiratory disease syndrome (SARS) is not fully understood. One case-control study has reported an association between susceptibility to SARS and mannan-binding lectin (MBL) in China. As the downstream protein of MBL, variants of the MBL-associated serine protease-2 (MASP2) gene may be associated with SARS coronavirus (SARS-CoV) infection in the same population.

Product Category

Recombinant Protein

Target

MASP2
Synonyms MAP19; MASP-2; MASP1P1; sMap
Accession

Species

Human

Amino Acid Range

Gly288-Phe686
Molecular Weight The protein has a predicted MW of 46.9 kDa. Due to autocatalytic cleavage, the protein migrates to 18 kDa&25-30 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human MASP2, His Tag at 0.5ug/ml (100ul/Well). Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test). The affinity constant of 3.08 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Express System

E.coli
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 20mM Tris, 200mM NaCl (pH 9.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Anti-MASP2 Antibody captured on CM5 Chip via Protein A can bind Human MASP2, His Tag with an affinity constant of 3.08 nM as determined in SPR assay (Biacore T200).
Immobilized Human MASP2, His Tag at 0.5ug/ml (100ul/Well). Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test).
Human MASP2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

You may also be interested in