- Express System: HEK293
- Product Tag: C-His
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is > 10000 pmol/min/µg.
- Amino Acid Range: Ile450-Val721
-
Other Available Proteins:
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Human MASP3 Protein (MSP-HM113) Summary
Product Description |
Recombinant Human MASP3 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ile450-Val721. |
Product Background |
Mannose-binding lectin-associated serine protease-1 (also named MASP3), a multifunctional serine protease, plays an important role in innate immunity which is capable of activating the lectin pathway of the complement system and also triggering coagulation cascade system. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human MASP3 Protein (MSP-HM113) Specifications/Details
Protein |
MASP3 |
Synonyms |
MASP-3; MASP-1; MASP1; RaRF; Serine protease 5; CRARF; CRARF1; PRSS5; |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ile450-Val721 |
Molecular Weight |
The protein has a predicted MW of 31.10 kDa. Due to glycosylation, the protein migrates to 43-53 kDa based on Bis-Tris PAGE result. |
Activity |
Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is > 10000 pmol/min/µg. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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