Product Description |
Recombinant Human Mature TGF beta 1 Protein is expressed from HEK293 without tag.It contains Ala279-Ser390. |
Product Background |
TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition.Transforming growth factor beta-1 is multifunctional protein that regulates the growth and differentiation of various cell types and is involved in various processes, such as normal development, immune function, microglia function and responses to neurodegeneration. |
Product Category |
Recombinant Protein |
Target |
Mature TGF beta 1 |
Synonyms |
CEDLAP; DPD1; TGF beta1; TGFB; TGFB1; TGFbeta; TGF-beta-1 |
Accession |
|
Species |
Human |
Amino Acid Range |
Ala279-Ser390 |
Molecular Weight |
The protein has a predicted MW of 13.2 kDa (monomer). Due to glycosylation, the protein migrates to 14-15 kDa (monomer) under reduced (R) condition and 26-30 kDa (dimer) under Non reducing (N) condition based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Mature TGF beta 1, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.35 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
No Tag |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 50mM Glycine 150mM NaCl (pH 2.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 4mM HCl. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human TGFBR1, mFc Tag captured on CM5 Chip via Protein A can bind Human Mature TGF beta 1, No Tag with an affinity constant of 0.35 uM as determined in SPR assay (Biacore T200). |
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Immobilized Human Mature TGF beta 1, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). |
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Human Mature TGF beta 1 on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%. |
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Measured by its ability to inhibit cell proliferation of Mv-1-lu mink lung epithelial cells. The ED50-for this effect is typically 0.1-0.2 ng/mL. |
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