Human Mature TGF beta 1 Protein (TG1-HM00M)

  • Express System: Expi293
  • Product Tag: No Tag
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human Mature TGF beta 1, No Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 8ng/ml determined by ELISA. The affinity constant of 0.35 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ala279-Ser390

Catalog: TG1-HM00M

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Product Description Recombinant Human Mature TGF beta 1 Protein is expressed from Expi293 without tag.It contains Ala279-Ser390.
Product Category Recombinant Protein
Target Mature TGF beta 1
Synonyms CEDLAP; DPD1; TGF beta1; TGFB; TGFB1; TGFbeta; TGF-beta-1
Gene/Protein ID P01137
Species Human
Exact Sequence Ala279-Ser390
Molecular Weight The protein has a predicted MW of 13.2 kDa. Due to glycosylation, the protein migrates to 14-15 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human Mature TGF beta 1, No Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 8ng/ml determined by ELISA. The affinity constant of 0.35 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag No Tag
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in 50mM Glycine 150mM NaCl (pH 2.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM Glycine 150mM NaCl (pH 2.5).
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human TGFBR1, mFc Tag captured on CM5 Chip via Protein A can bind Human Mature TGF beta 1, No Tag with an affinity constant of 0.35 μM as determined in SPR assay (Biacore T200).
Immobilized Human Mature TGF beta 1, No Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 8ng/ml determined by ELISA.
Human Mature TGF beta 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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