Human Mature TGF beta 1 Protein (TG1-HM00M)
Catalog: TG1-HM00M
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Product Description | Recombinant Human Mature TGF beta 1 Protein is expressed from Expi293 without tag.It contains Ala279-Ser390. |
Product Category | Recombinant Protein |
Target | Mature TGF beta 1 |
Synonyms | CEDLAP; DPD1; TGF beta1; TGFB; TGFB1; TGFbeta; TGF-beta-1 |
Gene/Protein ID | P01137 |
Species | Human |
Exact Sequence | Ala279-Ser390 |
Molecular Weight | The protein has a predicted MW of 13.2 kDa. Due to glycosylation, the protein migrates to 14-15 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human Mature TGF beta 1, No Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 8ng/ml determined by ELISA. The affinity constant of 0.35 μM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag | No Tag |
Express System | Expi293 |
Purity | > 95% as determined by Tris-Bis PAGE |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in 50mM Glycine 150mM NaCl (pH 2.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM Glycine 150mM NaCl (pH 2.5). |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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