Name: Human Mature TGF beta 1 Protein (TG1-HM00M)
Brand: KactusBio
SKU: TG1-HM00M-100UG
Price: 297.0 USD
Availability: InStock

ELISA binding curve of human mature TGF beta 1 (no tag) with TGF-beta RII-mFc confirming bioactivity and dose-dependent interaction.

Human Mature TGF beta 1 Protein (TG1-HM00M)

Human TGFBR1, mFc Tag captured on CM5 Chip via Protein A can bind Human Mature TGF beta 1, No Tag with an affinity constant of 0.35 uM as determined in SPR assay (Biacore T200).

Immobilized Human Mature TGF beta 1, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).

Human Mature TGF beta 1 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.

Measured by its ability to inhibit cell proliferation of Mv-1-lu mink lung epithelial cells. The ED50-for this effect is typically 0.1-0.2 ng/mL.

Express System: HEK293
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human Mature TGF beta 1, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.35 uM as determined in SPR assay (Biacore T200).
Amino Acid Range: Ala279-Ser390

Catalog #TG1-HM00M

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Human Mature TGF beta 1 Protein (TG1-HM00M) Summary

Product Description	Recombinant Human Mature TGF beta 1 Protein is expressed from HEK293 without tag.It contains Ala279-Ser390.
Product Background	TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition.Transforming growth factor beta-1 is multifunctional protein that regulates the growth and differentiation of various cell types and is involved in various processes, such as normal development, immune function, microglia function and responses to neurodegeneration.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human Mature TGF beta 1 Protein (TG1-HM00M) Specifications/Details

Protein	Mature TGF beta 1
Synonyms	CEDLAP; DPD1; TGFB1; TGF-β1; TGF-beta 1
Accession	P01137
Species	Human
Biotinylated	no
Amino Acid Range	Ala279-Ser390
Molecular Weight	The protein has a predicted MW of 13.2 kDa (monomer). Due to glycosylation, the protein migrates to 14-15 kDa (monomer) under reduced (R) condition and 26-30 kDa (dimer) under Non reduced (N) condition based on Bis-Tris PAGE result.
Activity	Immobilized Human Mature TGF beta 1, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.35 uM as determined in SPR assay (Biacore T200).
Product Tag	No Tag
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 50mM Glycine 150mM NaCl (pH 2.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in 4mM HCL. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human TGFBR1, mFc Tag captured on CM5 Chip via Protein A can bind Human Mature TGF beta 1, No Tag with an affinity constant of 0.35 uM as determined in SPR assay (Biacore T200).
	Immobilized Human Mature TGF beta 1, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).
	Human Mature TGF beta 1 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.
	Measured by its ability to inhibit cell proliferation of Mv-1-lu mink lung epithelial cells. The ED50-for this effect is typically 0.1-0.2 ng/mL.