Product Description |
Recombinant Human Mature TGF beta 2 Protein is expressed from HEK293 without tag. It contains Ala303-Ser414. |
Product Background |
Transforming growth factor beta(2) (TGF-beta(2)), a growth regulator of human lens epithelial cells (HLECs), also regulates the death of these cells. TGF-beta(2)-induced apoptosis in HLECs was preceded by an induction of reactive oxygen species (ROS) and a decrease in glutathione in the intracellular content, indicating that this factor induces oxidative stress in HLECs. |
Product Category |
Recombinant Protein |
Target |
Mature TGF beta 2 |
Synonyms |
TGFB2; TGFBR2; TGFR2; TbetaR-II; TGFβR2; BB105277; TGF-beta 2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala303-Ser414 |
Molecular Weight |
The protein has a predicted MW of 12.7 kDa. Due to glycosylation, the protein migrates to 13-15 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Mature TGF beta 2, No Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human TGF-beta RII, mFc Tag with the EC50 of 0.28ug/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
No Tag |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 4mM HCl. Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 4mM HCl. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human Mature TGF beta 2, No Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human TGF-beta RII, mFc Tag with the EC50 of 0.28ug/ml determined by ELISA (QC Test). |
|
|
Human Mature TGF beta 2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
Measured by its ability to inhibit the IL-4-dependent proliferation of TF-1 cells. The ED50 for this effect is 0.1 - 1 ng/ml (QC Test). |
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