Human Mature TGF beta 2 Protein (TG2-HM00M)

Express System: HEK293
Product Tag: No Tag
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human Mature TGF beta 2, No Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human TGF-beta RII, mFc Tag with the EC50 of 0.40ug/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ala303-Ser414

Catalog: TG2-HM00M

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Product Description	Recombinant Human Mature TGF beta 2 Protein is expressed from HEK293 without tag. It contains Ala303-Ser414.
Product Background	Transforming growth factor beta(2) (TGF-beta(2)), a growth regulator of human lens epithelial cells (HLECs), also regulates the death of these cells. TGF-beta(2)-induced apoptosis in HLECs was preceded by an induction of reactive oxygen species (ROS) and a decrease in glutathione in the intracellular content, indicating that this factor induces oxidative stress in HLECs.
Product Category	Recombinant Protein

Target	Mature TGF beta 2
Synonyms	TGFB2; TGFBR2; TGFR2; TbetaR-II; TGFβR2; BB105277; TGF-beta 2
Gene/Protein ID	P61812-1
Species	Human
Exact Sequence	Ala303-Ser414
Molecular Weight	The protein has a predicted MW of 12.7 kDa. Due to glycosylation, the protein migrates to 13-15 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human Mature TGF beta 2, No Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human TGF-beta RII, mFc Tag with the EC50 of 0.40ug/ml determined by ELISA. See testing image for detail.
Product Tag	No Tag
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 4mM HCl. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 4mM HCl.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human Mature TGF beta 2, No Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human TGF-beta RII, mFc Tag with the EC50 of 0.40ug/ml determined by ELISA.
	Measured by its ability to inhibit the IL-4-dependent proliferation of TF-1 cells. The ED50 for this effect is 0.1 - 1 ng/ml.
	Human Mature TGF beta 2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.