Human Mature TGF beta 3 Protein (TG3-HM00M)

Express System: HEK293
Product Tag: No Tag
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human Mature TGF beta 3, No Tag at 1ug (100ul/Well) on the plate. Dose response curve for Human TGF-beta RII, hFc Tag with the EC50 of 15.3ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ala301-Ser412

Catalog: TG3-HM00M

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Product Description	Recombinant Human Mature TGF beta 3 Protein is expressed from HEK293 without tag.It contains Ala301-Ser412.
Product Background	Transforming growth factor-beta 3 is a cytokine which is involved in cell growth regulation and differentiation, stimulation of extracellular matrix and modulation of immune responses. TGF beta 3 RNA is expressed in a diverse array of tissues including perichondrium, bone, intervertebral discs, mesenteries, pleura, heart, lung, palate, and amnion, as well as in central nervous system (CNS) structures such as the meninges, choroid plexus and the olfactory bulbs. Furthermore, in several organ systems, TGF beta 3 transcripts are expressed during periods of active morphogenesis suggesting that the protein may be an important factor for the growth and differentiation of many embryonic tissues.
Product Category	Recombinant Protein

Target	Mature TGF beta 3
Synonyms	TGF-beta-3; LAP; TGFB3; ARVD; ARVD1; RNHF
Gene/Protein ID	P10600-1
Species	Human
Exact Sequence	Ala301-Ser412
Molecular Weight	The protein has a predicted MW of 12.7 kDa. Due to glycosylation, the protein migrates to 13-15 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human Mature TGF beta 3, No Tag at 1ug (100ul/Well) on the plate. Dose response curve for Human TGF-beta RII, hFc Tag with the EC50 of 15.3ng/ml determined by ELISA. See testing image for detail.
Product Tag	No Tag
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 4mM HCl. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 4mM HCl.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human Mature TGF beta 3, No Tag at 1ug (100ul/Well) on the plate. Dose response curve for Human TGF-beta RII, hFc Tag with the EC50 of 15.3ng/ml determined by ELISA.
	Measured by its ability to inhibit the IL-4-dependent proliferation of TF-1 cells. The ED50 for this effect is 10-80 pg/ml.
	Human Mature TGF beta 3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.