Product Description |
Recombinant Human Mature TGF beta 3 Protein is expressed from HEK293 without tag.It contains Ala301-Ser412. |
Product Background |
Transforming growth factor-beta 3 is a cytokine which is involved in cell growth regulation and differentiation, stimulation of extracellular matrix and modulation of immune responses. TGF beta 3 RNA is expressed in a diverse array of tissues including perichondrium, bone, intervertebral discs, mesenteries, pleura, heart, lung, palate, and amnion, as well as in central nervous system (CNS) structures such as the meninges, choroid plexus and the olfactory bulbs. Furthermore, in several organ systems, TGF beta 3 transcripts are expressed during periods of active morphogenesis suggesting that the protein may be an important factor for the growth and differentiation of many embryonic tissues. |
Product Category |
Recombinant Protein |
Target |
Mature TGF beta 3 |
Synonyms |
TGF-beta-3; LAP; TGFB3; ARVD; ARVD1; RNHF |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala301-Ser412 |
Molecular Weight |
The protein has a predicted MW of 12.7 kDa. Due to glycosylation, the protein migrates to 13-15 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Mature TGF beta 3, No Tag at 1ug (100ul/Well) on the plate. Dose response curve for Human TGF-beta RII, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
No Tag |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 4mM HCl. Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 4mM HCl. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human Mature TGF beta 3, No Tag at 1ug (100ul/Well) on the plate. Dose response curve for Human TGF-beta RII, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). |
|
|
Human Mature TGF beta 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
Measured by its ability to inhibit the IL-4-dependent proliferation of TF-1 cells. The ED50 for this effect is 10-80 pg/ml (QC Test). |
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