Product Description |
Recombinant Human MDL-1/CLEC5A Protein is expressed from HEK293 with His tag at the N-terminus.It contains Pro28-Lys188. |
Product Background |
In C. elegans, increased lifespan in daf-2 insulin/IGF-1 receptor mutants is accompanied by up-regulation of the MDL-1 Mad basic helix-loop-helix leucine zipper transcription factor.MDL-1, like its mammalian orthologs, is an inhibitor of cell proliferation and growth that slows progression of an age-related pathology in C. elegans (uterine tumors). In addition, intestine-limited rescue of mdl-1 increased lifespan but not to wild type levels. Thus, mdl-1 likely acts both in the intestine and the germline to influence age-related mortality. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
MDL-1/CLEC5A |
Synonyms |
MDL-1; CLECSF5; MDL1; CLEC5A; DAP12-associating lectin 1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Pro28-Lys188 |
Molecular Weight |
The protein has a predicted MW of 19.49 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human MDL-1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MDL-1 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Immobilized Human MDL-1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MDL-1 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. |
|
|
Human MDL-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human MDL-1 is greater than 95% as determined by SEC-HPLC. |
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
More from Antibody Drug Discovery Proteins
Recently viewed
