Human MDL-1/CLEC5A Protein (CLE-HM25A)

  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human MDL-1, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-MDL-1 Antibody, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Tyr26-Lys188

Catalog: CLE-HM25A

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Product Description Recombinant Human MDL-1/CLEC5A Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Tyr26-Lys188.

Product Background

In C. elegans, increased lifespan in daf-2 insulin/IGF-1 receptor mutants is accompanied by up-regulation of the MDL-1 Mad basic helix-loop-helix leucine zipper transcription factor.MDL-1, like its mammalian orthologs, is an inhibitor of cell proliferation and growth that slows progression of an age-related pathology in C. elegans (uterine tumors). In addition, intestine-limited rescue of mdl-1 increased lifespan but not to wild type levels. Thus, mdl-1 likely acts both in the intestine and the germline to influence age-related mortality.

Product Category

Recombinant Protein

Target

MDL-1/CLEC5A
Synonyms MDL-1; CLECSF5; MDL1; CLEC5A; DAP12-associating lectin 1
Accession

Species

Human

Amino Acid Range

Tyr26-Lys188
Molecular Weight The protein has a predicted MW of 46 kDa. Due to glycosylation, the protein migrates to 63-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human MDL-1, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-MDL-1 Antibody, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA. See testing image for detail.

Product Tag

N-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human MDL-1, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-MDL-1 Antibody, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA.
The purity of Human MDL-1 is greater than 95% as determined by SEC-HPLC.
Human MDL-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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