Human MDL-1/CLEC5A Protein (CLE-HM25A)

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human MDL-1, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-MDL-1 Antibody, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Tyr26-Lys188

Catalog: CLE-HM25A

Order now get it on

Bulk Quote | Test Sample

Product Description	Recombinant Human MDL-1/CLEC5A Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Tyr26-Lys188.
Product Background	In C. elegans, increased lifespan in daf-2 insulin/IGF-1 receptor mutants is accompanied by up-regulation of the MDL-1 Mad basic helix-loop-helix leucine zipper transcription factor.MDL-1, like its mammalian orthologs, is an inhibitor of cell proliferation and growth that slows progression of an age-related pathology in C. elegans (uterine tumors). In addition, intestine-limited rescue of mdl-1 increased lifespan but not to wild type levels. Thus, mdl-1 likely acts both in the intestine and the germline to influence age-related mortality.
Product Category	Recombinant Protein

Target	MDL-1/CLEC5A
Synonyms	MDL-1; CLECSF5; MDL1; CLEC5A; DAP12-associating lectin 1
Gene/Protein ID	Q9NY25-1
Species	Human
Exact Sequence	Tyr26-Lys188
Molecular Weight	The protein has a predicted MW of 46 kDa. Due to glycosylation, the protein migrates to 63-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human MDL-1, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-MDL-1 Antibody, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human MDL-1, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-MDL-1 Antibody, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA.
	The purity of Human MDL-1 is greater than 95% as determined by SEC-HPLC.
	Human MDL-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.