Product Description |
Recombinant Human MDL-1/CLEC5A Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Tyr26-Lys188. |
Product Background |
In C. elegans, increased lifespan in daf-2 insulin/IGF-1 receptor mutants is accompanied by up-regulation of the MDL-1 Mad basic helix-loop-helix leucine zipper transcription factor.MDL-1, like its mammalian orthologs, is an inhibitor of cell proliferation and growth that slows progression of an age-related pathology in C. elegans (uterine tumors). In addition, intestine-limited rescue of mdl-1 increased lifespan but not to wild type levels. Thus, mdl-1 likely acts both in the intestine and the germline to influence age-related mortality. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
MDL-1/CLEC5A |
Synonyms |
MDL-1; CLECSF5; MDL1; CLEC5A; DAP12-associating lectin 1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Tyr26-Lys188 |
Molecular Weight |
The protein has a predicted MW of 46 kDa. Due to glycosylation, the protein migrates to 63-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human MDL-1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-MDL-1 Antibody, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Human MDL-1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-MDL-1 Antibody, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA. |
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Human MDL-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human MDL-1 is greater than 95% as determined by SEC-HPLC. |
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