• Express System: HEK293
  • Product Tag: N-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human MDL-1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-MDL-1 Antibody, hFc Tag with the EC50 of 35.8ng/ml determined by ELISA.
  • Amino Acid Range: Tyr26-Lys188
Catalog #CLE-HM25A
Price
Regular price $33000
Regular price Sale price $33000
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Size
Avantor VWR Fisher Scientific

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Human MDL-1/CLEC5A Protein (CLE-HM25A) Summary

Product Description

 Recombinant Human MDL-1/CLEC5A Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Tyr26-Lys188.

Product Background

 In C. elegans, increased lifespan in daf-2 insulin/IGF-1 receptor mutants is accompanied by up-regulation of the MDL-1 Mad basic helix-loop-helix leucine zipper transcription factor.MDL-1, like its mammalian orthologs, is an inhibitor of cell proliferation and growth that slows progression of an age-related pathology in C. elegans (uterine tumors). In addition, intestine-limited rescue of mdl-1 increased lifespan but not to wild type levels. Thus, mdl-1 likely acts both in the intestine and the germline to influence age-related mortality.

Human MDL-1/CLEC5A Protein (CLE-HM25A) Specifications/Details

Protein
 MDL-1/CLEC5A
Synonyms
 MDL-1; CLECSF5; MDL1; CLEC5A; DAP12-associating lectin 1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Tyr26-Lys188
Molecular Weight
 The protein has a predicted MW of 46 kDa. Due to glycosylation, the protein migrates to 63-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human MDL-1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-MDL-1 Antibody, hFc Tag with the EC50 of 35.8ng/ml determined by ELISA.
Product Tag
 N-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS, 8% trehalose (pH 7.4).
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human MDL-1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-MDL-1 Antibody, hFc Tag with the EC50 of 35.8ng/ml determined by ELISA. Immobilized Human MDL-1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-MDL-1 Antibody, hFc Tag with the EC50 of 35.8ng/ml determined by ELISA.
Human MDL-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human MDL-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human MDL-1 is greater than 95% as determined by SEC-HPLC. The purity of Human MDL-1 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...