Human MFAP4 Protein (MAP-HM104)

Express System: HEK293
Product Tag: N-His-Flag
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human MFAP4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MFAP4 Antibody, hFc Tag with the EC50 of 7.6ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Val22-Ala255

Catalog: MAP-HM104

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Product Description	Recombinant Human MFAP4 Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Val22-Ala255.
Product Background	Microfibril-associated glycoprotein 4 (MFAP4) is an extracellular matrix protein belonging to the fibrinogen-related protein superfamily. MFAP4 is produced by vascular smooth muscle cells and is highly enriched in the blood vessels of the heart and lung, where it is thought to contribute to the structure and function of elastic fibers. Genetic studies in humans have implicated MFAP4 in the pathogenesis of Smith-Magenis syndrome, in which patients present with multiple congenital abnormalities and mental retardation, as well as in the severe cardiac malformation left-sided congenital heart disease.
Product Category	Recombinant Protein

Target	MFAP4
Synonyms	Microfibril-associated glycoprotein 4；Mfap4
Gene/Protein ID	P55083-1
Species	Human
Exact Sequence	Val22-Ala255
Molecular Weight	The protein has a predicted MW of 28.6 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human MFAP4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MFAP4 Antibody, hFc Tag with the EC50 of 7.6ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His-Flag
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human MFAP4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MFAP4 Antibody, hFc Tag with the EC50 of 7.6ng/ml determined by ELISA.
	Human MFAP4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.