• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human MICA*002:01:04, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.22ug/ml determined by ELISA (QC Test).
  • Amino Acid Range: Glu24-Thr309
Catalog #MIC-HM104
Price
Regular price $34650
Regular price Sale price $34650
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Size
Avantor VWR Fisher Scientific

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Human MICA*002:01:04 Protein (MIC-HM104) Summary

Product Description

 Recombinant Human MICA*002:01:04 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Glu24-Thr309.

Product Background

 MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig‑like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin..

Human MICA*002:01:04 Protein (MIC-HM104) Specifications/Details

Protein
 MICA
Synonyms
 C5NT78; MICA; MICA*002:01:04
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Glu24-Thr309
Molecular Weight
 The protein has a predicted MW of 34.38 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human MICA*002:01:04, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.22ug/ml determined by ELISA (QC Test).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.； -80°C for 3 months after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human MICA*002:01:04, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.22ug/ml determined by ELISA (QC Test). Immobilized Human MICA*002:01:04, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.22ug/ml determined by ELISA (QC Test).
Human MICA*002:01:04 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human MICA*002:01:04 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human MICA*002:01:04 is greater than 95% as determined by SEC-HPLC. The purity of Human MICA*002:01:04 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...