Human MICA*009:01:11 Protein (MIC-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Human MICA*009:01:11, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.35ug/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Glu24-Thr309
Catalog #MIC-HM101
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Product Description
 Recombinant Human MICA*009:01:11 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Glu24-Thr309.
Product Background
 MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig‑like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin..
Product Category
 Recombinant Protein
Target
 MICA*009:01:11
Synonyms
 C5NT78; MICA; MICA*009:01:11
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Glu24-Thr309
Molecular Weight
 The protein has a predicted MW of 34.57 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human MICA*009:01:11, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.35ug/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human MICA*009:01:11, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.35ug/ml determined by ELISA (QC Test).
Human MICA*009:01:11 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human MICA*009:01:11 is greater than 95% as determined by SEC-HPLC.

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