Human MICA*012:08 Protein (MIC-HM106)
Human MICA*012:08 Protein (MIC-HM106)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human MICA*012:08, His Tag at 5ug/ml(100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 1.69ug/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Glu24-Thr309
Catalog #MIC-HM106
Regular price $346.50
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Product Description

Recombinant Human MICA*012:08 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Glu24-Thr309.

Product Background

MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig‑like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin..

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

MICA*012:08

Synonyms

C5NT78; MICA; MICA*012:08

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Glu24-Thr309

Molecular Weight

The protein has a predicted MW of 34.52 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human MICA*012:08, His Tag at 5ug/ml(100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 1.69ug/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human MICA*012:08, His Tag at 5ug/ml(100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 1.69ug/ml determined by ELISA (QC Test). Immobilized Human MICA*012:08, His Tag at 5ug/ml(100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 1.69ug/ml determined by ELISA (QC Test).
Human MICA*012:08 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human MICA*012:08 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human MICA*012:08 is greater than 95% as determined by SEC-HPLC. The purity of Human MICA*012:08 is greater than 95% as determined by SEC-HPLC.

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