Human MICA alpha 3 Protein (MIC-HM3AD)

Express System: HEK293
Product Tag: N-mFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human MICA alpha 3, mFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-MICA Antibody, hFc Tag with the EC50 of 0.10ug/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Arg105-Ser200

Catalog: MIC-HM3AD

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Product Description	Recombinant Human MICA alpha 3 Protein is expressed from HEK293 with mFc (IgG1) tag at the N-Terminus.It contains Arg105-Ser200.
Product Background	MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig‑like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin..
Product Category	Recombinant Protein

Target	MICA alpha 3
Accession	NP_001276081.1
Species	Human
Amino Acid Range	Arg105-Ser200
Molecular Weight	The protein has a predicted MW of 36.8 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human MICA alpha 3, mFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-MICA Antibody, hFc Tag with the EC50 of 0.10ug/ml determined by ELISA. See testing image for detail.
Product Tag	N-mFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human MICA alpha 3, mFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-MICA Antibody, hFc Tag with the EC50 of 0.10ug/ml determined by ELISA.
	The purity of Human MICA alpha 3 is greater than 95% as determined by SEC-HPLC.
	Human MICA alpha 3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.