Product Description |
Recombinant Human MICA alpha 3 Protein is expressed from HEK293 with mFc (IgG1) tag at the N-Terminus.It contains Arg105-Ser200. |
Product Background |
MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig‑like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin.. |
Product Category |
Recombinant Protein |
Target |
MICA alpha 3 |
Synonyms |
MICA; MIC-A; MICA alpha 3 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Arg105-Ser200 |
Molecular Weight |
The protein has a predicted MW of 36.8 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human MICA alpha 3, mFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-MICA Antibody, hFc Tag with the EC50 of 0.10ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-mFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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|
Immobilized Human MICA alpha 3, mFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-MICA Antibody, hFc Tag with the EC50 of 0.10ug/ml determined by ELISA. |
|
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Human MICA alpha 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human MICA alpha 3 is greater than 95% as determined by SEC-HPLC. |
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