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Human MICA alpha 3 Protein, Ultra Low Endotoxin (MIC-HM3AD-UL) Summary
Product Description |
Recombinant Human MICA alpha 3 Protein is expressed from HEK293 with mFc (IgG1) tag at the N-Terminus.It contains Arg105-Ser200. |
Product Background |
MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig‑like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin.. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human MICA alpha 3 Protein, Ultra Low Endotoxin (MIC-HM3AD-UL) Specifications/Details
Protein |
MICA |
Synonyms |
MICA; MIC-A; MICA alpha 3 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Arg105-Ser200 |
Molecular Weight |
The protein has a predicted MW of 36.8 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human MICA alpha 3, mFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-MICA Antibody, hFc Tag with the EC50 of 0.10ug/ml determined by ELISA (QC Test). |
Product Tag |
N-mFc |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human MICA alpha 3, mFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-MICA Antibody, hFc Tag with the EC50 of 0.10ug/ml determined by ELISA (QC Test). |
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Human MICA alpha 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human MICA alpha 3 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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