Human MICA Protein (MIC-HM20A)
Human MICA Protein (MIC-HM20A)
Human MICA Protein (MIC-HM20A)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human NKG2D, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human MICA, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.36 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Glu24-Gln308
Catalog #MIC-HM20A
Regular price $315.00
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Product Description
Recombinant Human MICA Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu24-Gln308.
Product Background
MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig‑like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin..
Product Category
Recombinant Protein
Target
MICA
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Glu24-Gln308
Molecular Weight
The protein has a predicted MW of 59.64 kDa. Due to glycosylation, the protein migrates to 75-85 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human NKG2D, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human MICA, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.36 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-hFc
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human MICA, hFc Tag captured on CM5 Chip via Protein A can bind Human NKG2D, His Tag with an affinity constant of 0.36 uM as determined in SPR assay (Biacore T200).
Immobilized Human NKG2D, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human MICA, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test).
Immobilized Human MICA, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-MICA Antibody, hFc Tag with the EC50 of 0.45ug/ml determined by ELISA.
Human MICA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human MICA is greater than 95% as determined by SEC-HPLC.

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