- Express System: HEK293
- Product Tag: C-His
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Human MICB*014:01:08, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.63ug/ml determined by ELISA (QC Test). See testing image for detail.
- Amino Acid Range: Ala23-Asp309
Product Description |
Recombinant Human MICB*014:01:08 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ala23-Asp309. |
Product Background |
MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB.MICB Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
MICB*014:01:08 |
Synonyms |
MICB; MIC-B; PERB11.2;MICB*014:01:08 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala23-Asp309 |
Molecular Weight |
The protein has a predicted MW of 34.33 kDa. Due to glycosylation, the protein migrates to 48-68 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human MICB*014:01:08, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.63ug/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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