ELISA binding curve showing interaction between human MICB*014:01:08 His-tagged protein and human NKG2D hFc-tagged receptor, with absorbance measured across protein concentrations.
Human MICB*014:01:08 Protein (MIC-HM108)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human MICB*014:01:08, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.63ug/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Ala23-Asp309
Catalog #MIC-HM108
Regular price $346.50
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Product Description

Recombinant Human MICB*014:01:08 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ala23-Asp309.

Product Background

MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB.MICB Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

MICB*014:01:08

Synonyms

MICB; MIC-B; PERB11.2;MICB*014:01:08

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Ala23-Asp309

Molecular Weight

The protein has a predicted MW of 34.33 kDa. Due to glycosylation, the protein migrates to 48-68 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human MICB*014:01:08, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.63ug/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human MICB*014:01:08, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.63ug/ml determined by ELISA (QC Test). Immobilized Human MICB*014:01:08, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.63ug/ml determined by ELISA (QC Test).
Human MICB*014:01:08 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human MICB*014:01:08 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human MICB*014:01:08 is greater than 95% as determined by SEC-HPLC. The purity of Human MICB*014:01:08 is greater than 95% as determined by SEC-HPLC.

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