Name: Human MICB Protein (MIC-HM20B)
Brand: KactusBio
SKU: MIC-HM20B-100UG
Price: 346.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human MICB, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-MICB Antibody, hFc Tag with the EC50 of 26.6ng/ml determined by ELISA (QC Test). The affinity constant of 95.15 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ala23-Gly298

Catalog #MIC-HM20B

Size

100UG

500UG

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Human MICB Protein (MIC-HM20B) Summary

Product Description	Recombinant Human MICB Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala23-Gly298.
Product Background	MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB.MICB Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human MICB Protein (MIC-HM20B) Specifications/Details

Protein	MICB
Synonyms	MICB; MIC-B; PERB11.2
Accession	Q29980-1
Species	Human
Biotinylated	no
Amino Acid Range	Ala23-Gly298
Molecular Weight	The protein has a predicted MW of 58.21 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human MICB, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-MICB Antibody, hFc Tag with the EC50 of 26.6ng/ml determined by ELISA (QC Test). The affinity constant of 95.15 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human MICB, hFc Tag captured on CM5 Chip via Protein A can bind Human NKG2D, His Tag with an affinity constant of 95.15 nM as determined in SPR assay (Biacore T200).
	Immobilized Human MICB, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-MICB Antibody, hFc Tag with the EC50 of 26.6ng/ml determined by ELISA (QC Test).
	Human MICB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human MICB is greater than 95% as determined by SEC-HPLC.