Human MICB Protein (MIC-HM40B)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human MICB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MICB Antibody, hFc Tag with the EC50 of 42.4ng/ml determined by ELISA. The affinity constant of 83.31 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ala23-Gly298

Catalog: MIC-HM40B

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Product Description	Recombinant Human MICB Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala23-Gly298.
Product Background	MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB.MICB Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
Product Category	Recombinant Protein

Target	MICB
Synonyms	MICB; MIC-B; PERB11.2
Gene/Protein ID	Q29980-1
Species	Human
Exact Sequence	Ala23-Gly298
Molecular Weight	The protein has a predicted MW of 34.4 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human MICB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MICB Antibody, hFc Tag with the EC50 of 42.4ng/ml determined by ELISA. The affinity constant of 83.31 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human NKG2D, hFc Tag captured on CM5 Chip via Protein A can bind Human MICB, His Tag with an affinity constant of 83.31 nM as determined in SPR assay (Biacore T200).
	Immobilized Human MICB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MICB Antibody, hFc Tag with the EC50 of 42.4ng/ml determined by ELISA.
	Immobilized Human MICB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.62ug/ml determined by ELISA (QC Test).
	The purity of Human MICB is greater than 95% as determined by SEC-HPLC.
	Human MICB on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.