Human MICB Protein (MIC-HM40B)
Human MICB Protein (MIC-HM40B)
Human MICB Protein (MIC-HM40B)
Human MICB Protein (MIC-HM40B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human MICB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MICB Antibody, hFc Tag with the EC50 of 42.4ng/ml determined by ELISA. The affinity constant of 83.31 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ala23-Gly298
Catalog #MIC-HM40B
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Product Description
 Recombinant Human MICB Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala23-Gly298.
Product Background
 MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB.MICB Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
Product Category
 Recombinant Protein
Target
 MICB
Synonyms
 MICB; MIC-B; PERB11.2
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala23-Gly298
Molecular Weight
 The protein has a predicted MW of 34.4 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human MICB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MICB Antibody, hFc Tag with the EC50 of 42.4ng/ml determined by ELISA. The affinity constant of 83.31 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human NKG2D, hFc Tag captured on CM5 Chip via Protein A can bind Human MICB, His Tag with an affinity constant of 83.31 nM as determined in SPR assay (Biacore T200).
Immobilized Human MICB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MICB Antibody, hFc Tag with the EC50 of 42.4ng/ml determined by ELISA.
Immobilized Human MICB, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.58ug/ml determined by ELISA (QC Test).
Human MICB on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human MICB is greater than 95% as determined by SEC-HPLC.
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