• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Amino Acid Range: Val21-Asp143
Catalog #MID-HM201
Price
Regular price $345.00
Regular price Sale price $345.00
/
Size
Avantor VWR Fisher Scientific

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Human Midkine Protein (MID-HM201) Summary

Product Description

Recombinant Human Midkine Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Val21-Asp143.

Product Background

Midkine is a heparin-binding growth factor, originally reported as the product of a retinoic acid-responsive gene during embryogenesis, but currently viewed as a multifaceted factor contributing to both normal tissue homeostasis and disease development. Midkine is abnormally expressed at high levels in various human malignancies and acts as a mediator for the acquisition of critical hallmarks of cancer, including cell growth, survival, metastasis, migration, and angiogenesis.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human Midkine Protein (MID-HM201) Specifications/Details

Protein
Midkine
Synonyms
MK; ARAP; MDK; MEK; MK1; MKARAP; NEGF2
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Val21-Asp143
Molecular Weight
The protein has a predicted MW of 39.36 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result.
Product Tag
C-hFc (IgG1)

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human Midkine on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Midkine on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...
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