Human MIF Protein (MIF-HM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Exact Sequence: Pro2-Ala115

Catalog: MIF-HM101

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Product Description Recombinant Human MIF Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Pro2-Ala115.

Product Background

 Macrophage migration inhibitory factor (MIF) is a pleiotropic cytokine with chemokine-like functions that increasingly is being studied in different aspects of cardiovascular disease. MIF was first identified as a proinflammatory and pro-survival mediator within the immune system, and a second structurally related MIF family member, D-dopachrome tautomerase (a.k.a. MIF-2), was reported recently.

Product Category

 Recombinant Protein

Target

 MIF

Gene/Protein ID

 P14174

Species

 Human

Exact Sequence

 Pro2-Ala115
Molecular Weight The protein has a predicted MW of 13.44 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.

Product Tag

 C-His

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human MIF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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