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Human MIF Protein (MIF-HM101) Summary
Product Description |
Recombinant Human MIF Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Pro2-Ala115. |
Product Background |
Macrophage migration inhibitory factor (MIF) is a pleiotropic cytokine with chemokine-like functions that increasingly is being studied in different aspects of cardiovascular disease. MIF was first identified as a proinflammatory and pro-survival mediator within the immune system, and a second structurally related MIF family member, D-dopachrome tautomerase (a.k.a. MIF-2), was reported recently. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human MIF Protein (MIF-HM101) Specifications/Details
Protein |
MIF |
Synonyms |
GIF; L-dopachrome isomerase; GLIF; MMIF; EC 5.3.2.1; EC 5.3.3.12 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Pro2-Ala115 |
Molecular Weight |
The protein has a predicted MW of 13.44 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human MIF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
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