Human MMP-8 Protein (MMP-HM108)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human MMP-8, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MMP-8 Antibody, hFc Tag with the EC50 of 1.93ug/ml determined by ELISA (QC Test). The affinity constant of 1.14 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Phe21-Gly467

Catalog: MMP-HM108

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Product Description Recombinant Human MMP-8 Protein is expressed from HEK293 with His tag at the C-Terminus. The protein needs to be activated by APMA to have hydrolytic activity.It contains Phe21-Gly467.

Product Background

Alteration of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) expression has been studied for various cardiac diseases, including dilated cardiomyopathy (DCM), with the significance of surrogate markers of extracellular matrix (ECM) remodeling. MMP-8 was identified only in myocardiocytes, while MMP-9 and TIMP-2 were present in both myocardiocytes and stroma, but with different intensity. The increasing intensity of MMP-8 and TIMP-2 immunoreactions was significantly associated with low HCS.

Product Category

Recombinant Protein

Target

MMP-8
Synonyms MMP-8; PMNL-CL; CLG1; MMP8; Collagenase 2; PMNL-CL; HNC

Gene/Protein ID

P22894

Species

Human

Exact Sequence

Phe21-Gly467
Molecular Weight The protein has a predicted MW of 52.2 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human MMP-8, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MMP-8 Antibody, hFc Tag with the EC50 of 1.93ug/ml determined by ELISA (QC Test). The affinity constant of 1.14 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 50mM Tris, 10mM CaCl2, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Anti-MMP-8 Antibody captured on CM5 Chip via Protein A can bind Human MMP-8, His Tag with an affinity constant of 1.14 uM as determined in SPR assay (Biacore T200).
Immobilized Human MMP-8, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MMP-8 Antibody, hFc Tag with the EC50 of 1.93ug/ml determined by ELISA (QC Test).
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is > 300 pmoles/min/ug (QC Test).
The purity of Human MMP-8 is greater than 95% as determined by SEC-HPLC.
Human MMP-8 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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