Human MMP-8 Protein (MMP-HM108)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human MMP-8, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MMP-8 Antibody, hFc Tag with the EC50 of 1.93ug/ml determined by ELISA (QC Test). The affinity constant of 1.14 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Phe21-Gly467

Catalog: MMP-HM108

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Product Description	Recombinant Human MMP-8 Protein is expressed from HEK293 with His tag at the C-Terminus. The protein needs to be activated by APMA to have hydrolytic activity.It contains Phe21-Gly467.
Product Category	Recombinant Protein

Target	MMP-8
Synonyms	MMP-8; PMNL-CL; CLG1; MMP8; Collagenase 2; PMNL-CL; HNC
Gene/Protein ID	P22894
Species	Human
Exact Sequence	Phe21-Gly467
Molecular Weight	The protein has a predicted MW of 52.2 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human MMP-8, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MMP-8 Antibody, hFc Tag with the EC50 of 1.93ug/ml determined by ELISA (QC Test). The affinity constant of 1.14 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 50mM Tris, 10mM CaCl2, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Anti-MMP-8 Antibody captured on CM5 Chip via Protein A can bind Human MMP-8, His Tag with an affinity constant of 1.14 uM as determined in SPR assay (Biacore T200).
	Immobilized Human MMP-8, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MMP-8 Antibody, hFc Tag with the EC50 of 1.93ug/ml determined by ELISA (QC Test).
	Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is > 300 pmoles/min/ug (QC Test).
	The purity of Human MMP-8 is greater than 95% as determined by SEC-HPLC.
	Human MMP-8 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.