Human MMP-8 Protein (pro form) (MMP-HM108)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human MMP-8, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-MMP-8 Antibody, hFc Tag with the EC50 of 3.5μg/ml determined by ELISA. The affinity constant of 1.14 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Phe21-Gly467

Catalog: MMP-HM108

$338.00
Size:

Order now get it on

Product Description Recombinant Human MMP-8 Protein is expressed from Expi293 with His tag at the C-terminal.It contains Phe21-Gly467.
Product Category Recombinant Protein
Target MMP-8
Synonyms MMP-8; PMNL-CL; CLG1; MMP8; Collagenase 2; PMNL-CL; HNC
Gene/Protein ID P22894
Species Human
Exact Sequence Phe21-Gly467
Molecular Weight The protein has a predicted MW of 52.2 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human MMP-8, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-MMP-8 Antibody, hFc Tag with the EC50 of 3.5μg/ml determined by ELISA. The affinity constant of 1.14 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in 50mM Tris,10mM CaCl2, 150mM NaCl (pH 7.5). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Anti-MMP-8 Antibody captured on CM5 Chip via Protein A can bind Human MMP-8, His Tag with an affinity constant of 1.14 μM as determined in SPR assay (Biacore T200).
Immobilized Human MMP-8, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-MMP-8 Antibody, hFc Tag with the EC50 of 3.5μg/ml determined by ELISA.
The purity of Human MMP-8 is greater than 95% as determined by SEC-HPLC.
Human MMP-8 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

You may also be interested in