Human MMP-9 Protein (pro form) (MMP-HM409)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human MMP-9, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ala20-Asp707

Catalog: MMP-HM409

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Product Description Recombinant Human MMP-9 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Ala20-Asp707.
Product Category Recombinant Protein
Target MMP-9
Synonyms MMP-9; GELB; CLG4B; MANDP2; Gelatinase B
Gene/Protein ID P14780
Species Human
Exact Sequence Ala20-Asp707
Molecular Weight The protein has a predicted MW of 79.3 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human MMP-9, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human MMP-9, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA.
The purity of Human MMP-9 is greater than 95% as determined by SEC-HPLC.
Human MMP-9 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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