Human MMP-9 Protein (MMP-HM409)
Human MMP-9 Protein (MMP-HM409)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human MMP-9, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala20-Asp707
Catalog #MMP-HM409
Regular price $315.00
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Product Description
Recombinant Human MMP-9 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. The protein needs to be activated by APMA to have hydrolytic activity.It contains Ala20-Asp707.
Product Background
Matrix metalloproteinase 9 (MMP9) contributes to this process and deficiencies in the MMP9 lead to impaired healing. Inappropriate expression of MMP9 also contributes to impaired re-epithelialization. Previously we demonstrated that FOXO1 was activated in wound healing but to higher levels in diabetic wounds. To address mechanisms of impaired re-epithelialization we examined MMP9 expression in vivo in full thickness dermal scalp wounds created in experimental K14.
Product Category
Recombinant Protein
Target
MMP-9
Synonyms
MMP-9; GELB; CLG4B; MANDP2; Gelatinase B
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ala20-Asp707
Molecular Weight
The protein has a predicted MW of 79.3 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human MMP-9, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human MMP-9, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA.
Human MMP-9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human MMP-9 is greater than 95% as determined by SEC-HPLC.

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