Human MMP-9 Protein (pro form) (MMP-HM209)

Immobilized Human MMP-9, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 13.6ng/ml determined by ELISA.

Human MMP-9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human MMP-9 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human MMP-9, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 13.6ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is > 1000 pmol/min/µg.
Amino Acid Range: Ala20-Asp707

Catalog #MMP-HM209

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Human MMP-9 Protein (pro form) (MMP-HM209) Summary

Product Description

Recombinant Human MMP-9 Protein (pro form) is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Ala20-Asp707.

Product Background

Matrix metalloproteinase 9 (MMP9) contributes to this process and deficiencies in the MMP9 lead to impaired healing. Inappropriate expression of MMP9 also contributes to impaired re-epithelialization. Previously we demonstrated that FOXO1 was activated in wound healing but to higher levels in diabetic wounds. To address mechanisms of impaired re-epithelialization we examined MMP9 expression in vivo in full thickness dermal scalp wounds created in experimental K14.

Human MMP-9 Protein (pro form) (MMP-HM209) Specifications/Details

Protein	MMP-9
Synonyms	MMP-9; GELB; CLG4B; MANDP2; Gelatinase B
Accession	P14780
Species	Human
Biotinylated	no
Amino Acid Range	Ala20-Asp707
Molecular Weight	The protein has a predicted MW of 102.31 kDa. Due to glycosylation, the protein migrates to 120-140 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human MMP-9, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 13.6ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is > 1000 pmol/min/µg.
Product Tag	C-hFc (IgG1)
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 50mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human MMP-9, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 13.6ng/ml determined by ELISA.
	Human MMP-9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human MMP-9 is greater than 95% as determined by SEC-HPLC.